Zebularine联合丁酸钠对结肠癌细胞系LOVO增殖、凋亡以及P16基因表达的影响  

Effects of Zebularine and sodium butyrate on cell growth,apoptosis and expression of P16 mRNA in human colon cancer cell line LOVO

作　　者：占义军[1] 邓涛[1] 李红艳[1] 万新月[1] 皮小雨[1] 

机构地区：[1]武汉大学人民医院消化内科,湖北武汉430060

出　　处：《胃肠病学和肝病学杂志》2012年第6期521-524,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨Zebularine和丁酸钠对结肠癌LOVO细胞增殖、凋亡及P16基因表达的影响。方法培养人结肠癌细胞系LOVO,分为4组:阴性对照组、Zebularine组、丁酸钠组、Zebularine+丁酸钠组。四甲基偶氮唑蓝(MTT)法检测细胞处理24 h、48 h、72 h后的增殖状况,Annexin V-FITC/PI双染法检测细胞凋亡,逆转录聚合酶链反应(RT-PCR)检测P16基因的表达。结果 Zebula-rine组和丁酸钠组LOVO细胞的抑制率和凋亡率与对照组相比显著增高,Zebularine+丁酸钠组细胞抑制率和凋亡率均显著高于单独用药组(P<0.05);Zebularine组和丁酸钠组P16 mRNA相对表达量显著高于对照组(P<0.05),Zebularine+丁酸钠组P16 mRNA相对表达量显著高于各单独用药组和对照组(P<0.05)。结论与单独用药相比,Zebularine联合丁酸钠可以明显促进P16 mRNA的表达,抑制结肠癌细胞生长。Objective To investigate the effect of Zebularine and sodium butyrate on cell growth, apoptosis and expression of P16 mRNA in human colon cancer cell line LOVO. Methods Human colon cancer cell lines LOVO were cultured and divided into Zebularine group, sodium butyrate group, Zebularine ＋ sodium butyrate group and control group. The cell growth rate was detected by MTT assay after 24 h, 48 h, 72 h of culture. The cell apoptosis was ana- lyzed by Annexin V-FITC/PI double staining. The expression of P16 mRNA was determined by reverse transcriptase pol- ymerase chain reaction （RT-PCR）. Results The cell growth inhibition rate and cell apoptosis rate significantly in- creased in Zebularine group and sodium butyrate group （P 〈 0.05） compared with control group, and the cell growth in- hibition rate and cell apoptosis rate of the Zebularine ＋ sodium butyrate group significantly increased compared with the Zebularine group and sodium butyrate group （P 〈0.05）. The relative expression of P16 mRNA of the Zebularine group and sodium butyrate group increased compared with the control, and the relative expression of P16 mRNA of the Zebu- larne ＋ sodium butyrate group significantly increased compared with Zebularine group and sodium butyrate group （P 〈 0.05）. Conclusion Compared with separate application of Zebularine or sodium butyrate, joint application could in- crease the expression of P16 mRNA and inhibit the growth of cell line LOVO.

关 键 词：ZEBULARINE 丁酸钠 P21 凋亡 结肠癌 

分 类 号：R735.35[医药卫生—肿瘤]