知母皂苷对脂多糖引起星形胶质细胞炎症因子释放的影响及机制  

Effect of SAaB on the release of inflammatory factors induced by lipopolysaccharide in cultured astrocytes in rats

作　　者：刘卓[1] 隋海娟[1] 闫恩志[1] 刘婉珠[2] 金英[1] 

机构地区：[1]辽宁医学院药理学教研室,辽宁锦州121001 [2]辽宁医学院机能实验中心,辽宁锦州121001

出　　处：《中国药理学通报》2012年第7期970-974,共5页Chinese Pharmacological Bulletin

基　　金：辽宁省教育厅创新团队项目(No LT2010064);辽宁省教育厅一般项目(No L2011143);辽宁医学院院内资助课题(No Y2010Z003)

摘　　要：目的研究知母皂苷(SAaB)对脂多糖(LPS)诱导的星形胶质细胞(AC)炎症因子释放的抑制作用及JNK信号传导通路对其的影响。方法实验设对照组、LPS组、SAaB组和阻断剂组。ELISA法和Griess法分别测定各组AC培养液中TNF-α和NO的含量;Western blot检测AC磷酸化JNK和磷酸化c-Jun蛋白表达水平的改变;免疫荧光染色法观察AC的磷酸化ATF-2蛋白表达水平。结果 AC在LPS(10mg.L-1)刺激下TNF-α和NO的分泌、磷酸化JNK1、磷酸化c-Jun和磷酸化ATF-2蛋白表达水平与正常对照组比较均明显增高。特异性JNK特异性阻断剂SP600125(10μmol.L-1)可明显抑制LPS引起的TNF-α和NO产生增加以及磷酸化ATF-2蛋白表达水平;SAaB(1、10、100μmol.L-1)则可明显降低TNF-α和NO产生,下调磷酸化JNK、磷酸化c-Jun和磷酸化ATF-2的蛋白表达水平。结论 SAaB能明显抑制LPS诱导的大鼠皮层AC炎症因子的释放,这种作用可能与其下调JNK信号转导通路有关。Aim To investigate whether Saponins from Anemarrhena asphdeloids Bge（SAaB） can protect cortical astrocytes from lipopolysaccharide（LPS）-in-duced release of inflammatory factors and to study the mechanisms responsible for this protective effect.Methods Cultured astrocytes were treated with LPS（10 mg·L-1） for 24 h,in the Absence or presence of SAaB（1,10 and 100 μmol·L-1） or the specific inhibitor of JNK SP600125（10 μmol·L-1）,and harvested.Tumor necrosis factor-α（TNF-α） and nitric oxide（NO） generation were analysed respectively by ELISA and Griess technology.Western blot was performed to observe the level of phospho-JNK and phospho-c-Jun in cultured astrocytes.Immunofluorescent technology was performed to observe the level of phospho-activating transcription factor-2（ATF-2） protein expression.Results LPS induced a significant increase in phospho-JNK and phospho-c-Jun protein expression without affecting total protein levels,and increased the expression of phospho-ATF-2 and the release of TNF-α and NO in cultured astrocytes in rats.SP600125 significantly inhibited LPS-induced increase in the release of TNF-α and NO.SAaB obviously inhibited LPS-induced increase in the release of TNF-α and NO and the expression of phospho-JNK,phospho-c-Jun and phospho-ATF-2 protein in cultured astrocytes.Conclusion This study indicates that SAaB can inhibit the release of TNF-α and NO induced by LPS in cultured astrocytes through the suppression of JNK signal transduction pathway activity.

关 键 词：知母皂苷 脂多糖 星形胶质细胞 炎症因子 C-JUN氨基末端激酶 转录因子C-JUN 活化转录因子-2 

分 类 号：R-332[医药卫生] R284.1