白香丹含药血清对大鼠海马神经元中5-羟色胺2C受体的表达及其下游信号通路中IP_3的影响  

Effect of Baixiangdan on the expression of 5-HTR2C and the concentration of IP_3 in neonate rat hippocampal neurons cultured in vitro

作　　者：柳新[1] 薛玲[1] 高杰[1] 魏盛[1] 谭倩[1] 苏云祥[1] 

机构地区：[1]山东中医药大学中医药经典理论教育部重点实验室,山东济南250355

出　　处：《中国药理学通报》2012年第7期1019-1023,共5页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)资助课题(No2011CB505102);国家自然科学基金重点项目(No30930110)

摘　　要：目的观察白香丹胶囊含药血清对原代海马神经元中5-羟色胺2C受体(5-HTR2C)蛋白表达水平及下游信号通路中IP3含量的影响,探讨其可能的中枢作用机制。方法采用情志刺激为主多因素造模法制备经前期综合征(PMS)肝气逆证大鼠模型,给予白香丹干预,制备大鼠含药血清,结合中药血清药理学,对体外培养的新生大鼠原代海马神经元进行不同血清干预,运用Western blot方法检测海马神经元中5-HTR2C蛋白表达水平,运用ELISA法检测各组海马神经细胞内IP3的含量。结果与正常血清组相比,PMS肝气逆模型血清组海马神经元的5-HTR2C蛋白表达水平和下游信号通路中IP3含量均明显升高(P<0.01,P<0.01),白香丹含药血清能够明显降低5-HTR2C蛋白表达水平和IP3的含量(P<0.01,P<0.01)。结论 PMS肝气逆证模型大鼠血清使海马神经元中5-HTR2C蛋白表达异常升高,同时,可引起第二信使IP3含量升高;白香丹胶囊可能通过降低5-HTR2C蛋白表达水平,改变其下游信号通路中IP3的含量而发挥疗效。Aim To explore the intervention mechanisms of baixiangdan capsule by observing the changes of the expression level of 5-HTR2C and the concentration of IP3 in neonate rat hippocampal neurons cultured in vitro.Methods Premenstrual syndrome（PMS） model rats with liver-qi invasion were induced by multiple emotional stimulations and baixiangdan capsule was administered after PMS induction.The medicated serum was used to neonate rat hippocampal neurons.The expression level of 5-HTR2C in the neurons was evaluated by Western blot and the concentration of IP3 was measured with the Elisa method.Results Compared with control group,both the protein expression of 5-HTR2C and the concentration of IP3 of PMS liver-qi invation group were significantly higher in neurons（P〈0.01,P〈0.01）.After treated with baixiangdan medicated serum,the expression of 5-HTR2C and the concentration of IP3 in hippocampal neurons were significantly improved（P〈0.01,P〈0.01）.Conclusions PMS model rats with liver-qi invasion serum can up-regulate the expression of 5-HTR2C in hippocampal neurons and increase the concentration of the second messenger IP3.Baixiangdan capsule might partially play its role in ameliorating PMS by regulating the expression of 5-HTR2C and changing the concentration of IP3.

关 键 词：白香丹胶囊 经前期综合征肝气逆证 大鼠 海马神经元 含药血清 5-HTR2C IP3 

分 类 号：R-332[医药卫生] R282.71