食管鳞癌患者血清中可溶性CD44v6和E-钙黏蛋白表达的临床意义  

Serum sCD44v6 and sE-cadherin levels in patients with esophageal squamous cell carcinoma

作　　者：何赢[1,2] 张军[1,2] 王书奎[1,2] 黄文斌[1,2] 陈兴国[1,2] 何帮顺[1,2] 赵有财[1,2] 王劲松[1,2] 

机构地区：[1]南京医科大学附属南京医院 [2]南京第一医院,江苏南京210006

出　　处：《浙江大学学报（医学版）》2012年第3期305-309,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：2008年度南京市医学科技发展基金资助项目(项目编号YKK08095)

摘　　要：目的:检测食管鳞癌患者血清中可溶性CD44v6(sCD44v6)和可溶性E-钙黏蛋白(sE-cadherin,sE-cad)的表达,探讨这2种可溶性黏附分子表达与食管鳞癌临床病理特征的关系以及这2种可溶性黏附分子在血清中表达的相关性。方法:采集65例食管鳞癌患者、32例糜烂性食管炎患者和35例正常食管者的血清,采用酶联免疫吸附法(ELISA)检测血清中sCD44v6、sE-cad的表达,并与其临床病理资料对照。结果:血清sCD44v6在食管鳞癌组表达水平明显高于正常食管组及糜烂性食管炎组(P<0.05),正常食管组与糜烂性食管炎组比较无统计学差异(P=0.566);血清sCD44v6水平与食管鳞癌临床病理特征无关。血清sE-cad水平在食管鳞癌组明显高于正常食管组及糜烂性食管炎组(P<0.05),正常食管组与糜烂性食管炎组比较无统计学差异(P=0.708);血清sE-cad水平与食管鳞癌临床病理特征无关。食管鳞癌患者血清中sCD44v6和sE-cad水平无显著相关性(r=-0.057,P=0.651)。结论:血清sCD44v6及sE-cad水平测定有望成为筛查食管鳞癌的血清学指标。Objective： To investigate the serum levels of sCD44v6 and sE-eadherin （sE-cad） in patients with esophageal squamous cell carcinoma. Methods： The serum samPles, were collected from 65 cases of esophageal squamous cell carcinoma, 32 cases of erosive esophagitis and 35 healthy subjects. Serum sCD44v6 and sE-cad levels were measured by enzyme linked immunosorbent assay （ELISA）. Results ： The mean levels of serum sCD44v6 and sE-cad in esophageal squamous cell carcinoma patients were significantly higher than those of erosive esophagitis patients and normal controls （ both P 〈 0.05 ） There was no significant difference in serum sCD4dv6 and sE-cad levels between erosive esophagitis patients normal controls （P = 0. 566 and P = 0. 708, respectively）. Serum sCD44v6 and sE-cad levels of esophageal cancer patients were not correlated with their clinicopathological features. Serum sCD44v6 level is not correlated with sE-cad level in squamous cell carcinoma patients （ P = 0.651 ）. Conclusion： Serum sCD44v6 and sE-cad might be a potential marker for screening of esophageal squamous cell carcinoma.

关 键 词：酶联免疫吸附测定 基因表达 钙黏着糖蛋白类/分析 食管肿瘤 肿瘤 鳞状细胞 抗原 CD44/分析 

分 类 号：R735.1[医药卫生—肿瘤]