Glucose-induced posttranslational activation of protein phosphatases PP2A and PP1 in yeast  

作　　者：Dries Castermans Ils Somers Johan Kriel Wendy Louwet Stefaan Wera Matthias Versele Veerle Janssens Johan M Thevelein 

机构地区：[1]Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KULeuven, Belgium [2]Department of Molecular Mi- crobiology, VIB, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Belgium [3]Protein Phosphorylation and Proteomics Labora- tory, Department of Molecular Cell Biology, Faculty of Medicine, KULeuven, B-3000 Leuven, Flanders, Belgium

出　　处：《Cell Research》2012年第6期1058-1077,共20页细胞研究（英文版）

摘　　要：The protein phosphatases PP2A and PP1 are major regulators of a variety of cellular processes in yeast and other eukaryotes. Here, we reveal that both enzymes are direct targets of glucose sensing. Addition of glucose to glucose- deprived yeast cells triggered rapid posttranslational activation of both PP2A and PP1. Glucose activation of PP2A is controlled by regulatory subunits Rtsl, Cdc55, Rrdl and Rrd2. It is associated with rapid carboxymethylation of the catalytic subunits, which is necessary but not sufficient for activation. Glucose activation of PP1 was fully dependent on regulatory subunits Regl and Shpl. Absence of Gacl, Glc8, Reg2 or Redl partially reduced activation while Pigl and Pig2 inhibited activation.

关 键 词：glucose sensing YEAST protein phosphatases PP1 PP2A 

分 类 号：Q257[生物学—细胞生物学] X520.322.5[环境科学与工程—环境工程]