梅花入骨丹多糖中蛋白含量的测定  

Determination of Protein Content in Polysaccharides from Bauhinia Championi(Benth.) Benth.

作　　者：蔡亮亮[1] 李西海[2] 余方荣[1] 陈加守[1] 李会婷[1] 郑春松[1] 徐慧凤[1] 庄怀山[2] 刘献祥[2] 叶蕻芝[2] 

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建中医药大学中西医结合研究院,福建福州350122

出　　处：《福建中医药大学学报》2012年第3期37-39,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省自然科学基金资助课题(201101035);福建省属高校科研专项资助课题(JK2009016);福建省中医药科研重点资助课题(WZZG0901);陈可冀中西医结合发展基金(CKJ2011003)

摘　　要：目的建立梅花入骨丹多糖中蛋白含量的测定方法。方法用水提醇沉法提取梅花入骨丹多糖,考马斯亮蓝法测定蛋白含量,比较Sevage法、木瓜蛋白酶-Sevge法、三氯乙酸-Sevage法、三氯乙酸-正丁醇法脱蛋白的效果。结果 Sevage法、木瓜蛋白酶-Sevage法、三氯乙酸-Se-vage法、三氯乙酸-正丁醇法蛋白去除率分别为46.21%、71.43%、81.12%、88.11%,多糖损失率分别为33.73%、18.51%、43.40%、28.15%。结论木瓜蛋白酶-Sevage法脱蛋白较好。Objective To determine the protein content in polysaccharides from Bauhinia Champi- oni （Benth.） Benth.. Methods The water extraction and alcohol precipitation method was adopted to obtain polysaccharide, Coomassie brilliant blue method was used to detect the protein content. The Sevage method, combined methods of papain and Sevage, combined methods of trichloroacetic acid and Sevage and combined methods of trichloroacetic acid and n-butanol were adopted to remove the protein respectively, and the effects of different methods were compared. Results The percentage of the removed protein for Sevage method, combined methods of papain and Sevage, combined methods of trichloroacetic acid and Sevage and combined methods of trichloroacetic acid and n-butanol were 46.21%, 71.43%, 81.12% and 88.11% respectively, and the loss rate of polysaccharides was 33.73%, 18.51%, 43.40% and 28.15% respectively. Conclusion The combined methods of papain and Sevage was the best for removing protein.

关 键 词：梅花入骨丹多糖 蛋白含量 脱蛋白 蛋白去除率 多糖损失率 

分 类 号：R284[医药卫生—中药学]