大鼠牙周炎牙龈卟啉单胞菌毒力调节基因变化  

Porphyromonas gingivalis vim A in experimental periodontitis in rats

作　　者：潘春玲[1] 谢韬[2] 赵戬[1] 潘亚萍[1] 钟鸣[3] 

机构地区：[1]中国医科大学口腔医学院牙周科辽宁省口腔医学研究所牙周病研究室,辽宁沈阳110002 [2]辽宁省疾病预防控制中心 [3]中国医科大学口腔医学院中心实验室

出　　处：《中国公共卫生》2012年第6期787-788,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(81070834)

摘　　要：目的观察大鼠实验性牙周炎发生发展过程中牙龈卟啉单胞菌毒力调节基因A(virulence modulatinggene A,vim A)相对含量的变化。方法采用正畸钢丝结扎双上颌第一磨牙颈部、局部牙龈剥离、细菌灌饲及高糖饮水的方法,建立大鼠实验性牙周炎模型。分别在4周和8周取上颌第一磨牙龈下菌斑,提取细菌DNA,应用PCR方法进行牙龈卟啉单胞菌vim A特异引物PCR扩增,应用SPSS 11.0统计软件包,分析大鼠牙周炎不同时段牙龈卟啉单胞菌、vim A相对含量变化。结果实验性牙周炎模型大鼠龈下菌斑中牙龈卟啉单胞菌4周和8周相对含量分别为66.3%和81.2%,明显高于对照组,8周组高于4周组;vim A相对含量在4周组和8周组分别为58.7%和62.4%,明显高于对照组,4周组和8周组vim A相对含量无明显差异。结论牙龈卟啉单胞菌vim A基因与牙周炎的发生密切相关,与牙周炎严重程度可能无关。Objective To examine the relative content changes of Porphyromonas gingivalis（P.gingivalis） inoculated to subgingival of first maxillary molars of the rats in different times and the changes of virulence modulating gene A（vim A） by establishing the rat periodontitis model.Methods We established a rat experimental periodontitis model by ligaturing orthodontic steel wire and feeding bacteria.The subgingival plaque was collected,then the DNA was extracted and amplified with PCR using specific primer.We analyzed the genetic changes of vim A in different stages of the rat periodontitis using SPSS11.0 with two independent t test.Results The relative contents of P.gingivalis of experimental group in 4 weeks and 8 weeks were significantly different and obviously higher than those of the control group,while the relative contents of vim A for both of the experimental group and healthy controls increased.Conclusion Vim A gene may relate to the pathogenicity of periodontitis,but have no direct effect on the severity of periodontal disease.

关 键 词：牙周炎 牙龈卟啉单胞菌 毒力调节基因A 

分 类 号：R781.4[医药卫生—口腔医学]