茶树花粉特异蛋白基因CsPSP的反义载体构建  

Antisense vector construction of CsPSP pollen specific protein gene from tea plant(Camellia sinensis L.)

作　　者：龚莹[1] 余梅[2] 江昌俊[1] 吴凡[2] 吉虎[2] 徐凯[2] 臧洁[2] 

机构地区：[1]安徽农业大学教育部和农业部茶叶生物化学与生物技术重点实验室,合肥230036 [2]安徽农业大学生命科学学院,合肥230036

出　　处：《安徽农业大学学报》2012年第3期397-400,共4页Journal of Anhui Agricultural University

基　　金：安徽高校省级自然科学研究重点项目计划(KJ2010A119)资助

摘　　要：以茶树龙井43品种的后期花蕾为试验材料,提取总RNA,然后进行RT-PCR得到cDNA第1链。同时,根据GenBank上登录的茶树花粉特异蛋白基因CsPSP(DQ887753)的序列,设计1对特异引物进行PCR反应。将扩增后的基因片段插入pBI121表达载体中,并测序。结果表明,插入pBI121载体的片段长度为318 bp,与已知序列进行比对后一致性达到99.02%,说明载体构建成功。In order to study the expression of the pollen-specific gene in tea plant, a pair of specific primers with double enzyme sites was designed to clone a cDNA fragment of CsPSP gene from Longjing43 by the method of RT-PCR, and then the CsPSP fragment was ligated with pBI121 vector and sequenced. The result indicates that the sequence has 99.02% similarity to CsPSP gene in GenBank.

关 键 词：茶树 CsPSP 反义载体 

分 类 号：S571.1[农业科学—茶叶生产加工]