生长停滞特异性基因产物6的2个单核苷酸多态性与缺血性脑卒中的相关性  

Relation between two single nucleotide polymorphisms of growth arrest-specific gene 6 and ischemic stroke

作　　者：高永俊[1] 李晓峰[1] 杨旭[2,3] 李新毅[4,5] 

机构地区：[1]山西医科大学第一医院神经内科,太原030001 [2]航天中心医院 [3]北京大学航天临床医学院神经内科 [4]山西医学科学院 [5]山西大医院神经内科

出　　处：《中华老年心脑血管病杂志》2012年第6期576-580,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的探讨生长停滞特异性基因产物6(growth arrest-specific gene 6,Gas 6)单核苷酸多态性(single nucleo-tide polymorphisms,SNP)与缺血性脑卒中(ischemic stroke,IS)及其亚型发生的相关性。方法选择IS患者119例作为病例组,同期随机抽取无心、脑及周围动脉粥样硬化史的健康体检者217例作为对照组。根据既往研究的阳性结果及HapMap数据库,选择Gas6 rs8191974及rs7400722位点分析。采用PCR-RFLP技术进行2个SNP基因分型。采用logistic多元回归分析IS及其亚型的危险因素。结果 2组2个SNP基因型(P=0.80、0.79)、等位基因频率(P=0.57、0.50)及不同模型基因频率(显性:P=0.93、0.58;隐性:P=0.51、0.58)比较,差异均无统计学意义;以连锁不平衡系数>0.5为判断标准,共构建4个单体型(G-C,G-T,A-C,A T),2组4个单体型分布频率差异均无统计学意义(P>0.05);校正相关危险因素后,差异仍无统计学意义(P>0.05)。结论 Gas6 rs8191 974及rs7400722位点基因型、等位基因频率及单体型与IS无明显相关性。Objective To study the relation of single nucleotide polymorphisms（SNP） of growth arrest-specific gene 6（Gas6） with ischemic stroke（IS） and its subtypes. Methods One hundred and nineteen IS patients served as a patient group and 217 subjects with no history of cardiac,cerebral and peripheral athrosclerosis served as a control group in this study. The rs8191974 and rs7400722 polymorphisms of Gas6,selected according to the positive findings in previous studies and in HapMap database,were detected by PCR-RFLP. Risk factors for IS and its subtypes were analyzed by multivariate logistic regression analysis. Results No significant difference was found in the 2 ingle nucleotide SNP, frequency of allele and Gas6 gene in different models, and in the 4 haplotypes（G-C, G-T, A-C, A-T） we established with the chain-imbalance factor 〉0. 05 as a judge criterion between the two groups, even after the related risk factors were corrected （P〉 0.05）. Conclusion The rs7400722 and rs8191974 polymorphisms of Gas6 and frequency of allele and haplotype are not significantly related with IS.

关 键 词：脑缺血 卒中 多态性 单核苷酸 基因型 基因频率 危险因素 

分 类 号：R743.3[医药卫生—神经病学与精神病学]