大鼠糖尿病肾病模型建立影响因素研究  

Influential factors on the establishment of experimental diabetic nephropathy in rats

作　　者：杨峰[1,2] 唐丽琴[2] 王凤玲[1,2] 朱玲娜[2,3] 蔡明[2,3] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所,安徽合肥230032 [2]安徽医科大学附属省立医院,安徽合肥230001 [3]安徽中医学院,安徽合肥230031

出　　处：《安徽医药》2012年第6期735-738,共4页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(No 81073109);国家自然科学基金资助项目(No 81102864);安徽省自然科学基金资助项目(No 090413106);安徽省卫生厅医学科研课题(No09C219)

摘　　要：目的采用高脂饲料喂养联合低剂量链脲菌素(STZ)制备糖尿病肾病(DN)大鼠模型,探讨高脂饲料喂养时间和STZ剂量对模型成模率和死亡率的影响,确定建立DN大鼠模型的最佳方案。方法雄性SD大鼠先给予高脂饲料喂养,诱发胰岛素抵抗,再给予不同剂量STZ腹腔注射,建立DN模型。结果高脂饲料4周+STZ 40 mg·kg-1和高脂6周+STZ 35 mg·kg-1制备的糖尿病大鼠模型,其血糖值、相对肾重、胶原蛋白和TGF-β1的表达和正常组比较都差异有显著性,但高脂饲料4周+STZ40 mg·kg-1的造模死亡率较高。结论高脂喂养6周后腹腔注射低剂量STZ 35 mg·kg-1制备的DN大鼠模型,成模率高,模型稳定。Objective To investigate the effect of the duration of high-fat-diet feeding and various doses of streptozotocin （STZ） on the model achievement ratio and mortality of high-fat-diet and streptozotocin-induced diabetic nephropathy （DN） rat model,and to determine the preferred plan for the establishment of DN rats model. Methods Male Sprague-Dawley rats （ n = 56） were fed high fat diet for four or six weeks to induce insuIin resistance and then injected with streptozotocin （STZ ,35 mg· kg^- 1 or 40 mg · kg^- l intraperitoneal） to es- tablish a rat model of DN. Results Fasting blood glucose, the kidney weight/body weight （KW/BW） ratio, collagen, transforming growth factor-β1 （TGF-β1） in DN rats which were fed high-fat-diet for four weeks plus STZ（40 mg · kg^-1 ） and fed high-fat-diet for six weeks plus STZ （35 mg ·kg^- 1） had statistically significant difference （P 〈 0.05 or P 〈 0.01 ） when compared with normal group rats. Howev- er,it presents a high mortality in high-fat-diet for four weeks plus STZ （40 mg ·kg^- 1 ）-induced DN rats. Conclusion The model rats that fed with high-fat-diet for six weeks followed by intraperitoneal injection of STZ（35mg ·kg^- 1 ） proves to be the optimal one.

关 键 词：糖尿病肾病 动物模型 链脲佐菌素 高脂饲料 大鼠 

分 类 号：R-332[医药卫生] R587.2R692.9