Survivin反义寡核苷酸诱导人胃癌细胞裸鼠皮下移植瘤细胞凋亡的研究  

Effects of Survivin antisense oligonucleotide on molecular mechanism of inducing human gastric transplantable tumor apoptosis

作　　者：王超群[1] 郑茂金[1] 梁栋[1] 周云[1] 

机构地区：[1]徐州医学院,江苏221002

出　　处：《齐齐哈尔医学院学报》2012年第10期1265-1267,共3页Journal of Qiqihar Medical University

摘　　要：目的研究survivin反义寡核苷酸(antisense oligodeoxynucleotide,ASODN)对人胃癌细胞裸鼠移植瘤生长的影响,并探讨其诱导人胃癌移植瘤凋亡的分子生物学机制。方法建立SGC-7901胃癌细胞裸鼠皮下移植瘤模型,随机分为4组,即survivin-ASODN+脂质体组、survivin-ASODN组、脂质体组和空白对照组(蒸馏水)。瘤体内隔日给药,停药后48小时处死裸鼠,剥离瘤组织,称取瘤重,计算抑瘤率。western-blot法检测各组凋亡相关蛋白survivin、Caspase-3蛋白的表达。结果 survivin-ASODN+脂质体组的移植瘤生长速度明显减慢,其抑瘤率为:33.19%。survivin-ASODN+脂质体组的survivin蛋白表达量明显低于对照组(P<0.05),Caspase-3蛋白表达高于对照组(P<0.05)。结论 survivin反义寡核苷酸转染胃癌移植瘤能够明显抑制肿瘤生长,其主要机制与降低survivin蛋白的表达,诱导Caspase-3高表达,从而促进肿瘤细胞凋亡有关。Objective To investigate the effects of Survivin antisense oligonucleotide on the subcu- taneous transplantable tumor of human gastric cell line in nude mice, and its molecular mechanism of in- ducing human gastric transplantable tumor apoptosis. Methods The subcutaneous transplantable tumor model of human gastric eareinoma in nude mice were established and then were divided at random into four groups ： survivin--ASODN -~ liposome group, survivin--ASODN group, liposome group and blank group. Then received every other day medication in tumor interior, executed nude mice after 48hours of drug withdrawal, took out the tumors and obtained the weight. And western-- blot detected the proteinie presentation of survivin, Caspase--3. Results Survivin--ASODNq- liposome group could ob- viously inhibit the growth of transplantable tumor with the tumor inhibitory rates of 33.19 ~. Western-- blot indicated：in survivin--ASODNq- liposome group the proteinic presentation of survivin （P 〈 0.05）, and the proteinic presentation of Caspase- 3 is higher than other blank group （P 〈 0.05）. Conclusions Survivin antisense oligonucleotide conuld suppress obviously the growth of the subcutane- ous transplantable tumor of human gastric cell line in nude mice, which maybe relative to suppress the proteinie presentation of survivin, thus the up--regulation of the presentation of Caspase-- 3 protein, thus suppress cell increment.

关 键 词：SURVIVIN 反以寡核苷酸 胃癌 移植瘤 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]