莱菔硫烷对膀胱癌细胞侵袭能力的影响及机制研究  

Effects of SFN on the Invasion of Human Bladder Cancer Cell and Its Mechanism

作　　者：冯雪[1] 李宝龙[2] 郜明明[1] 何灿霞[1] 潘萌[1] 黄睿[1] 王凤前[1] 单毓娟[1,3] 

机构地区：[1]哈尔滨医科大学,黑龙江哈尔滨150081 [2]黑龙江中医药大学药物安全性评价中心,黑龙江哈尔滨150040 [3]哈尔滨工业大学,黑龙江哈尔滨150006

出　　处：《中医药信息》2012年第3期99-101,共3页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金资助(81172648)

摘　　要：目的:探讨莱菔硫烷(sulforaphane,SFN)对膀胱癌细胞侵袭性及上皮-间质转化(epithelial-mesenchymal transition,EMT)影响和作用机制。方法:常规培养膀胱癌细胞株T24后,采用Transwell侵袭小室法观察SFN对T24细胞体外侵袭能力的影响;采用Western blot法检测SFN作用T24细胞后EMT标志蛋白的表达变化。结果:10μmol/L SFN作用24h后的T24细胞穿透能力显著下降,明显诱导EMT标志蛋白E-cadherin的表达增高并下调vimentin的表达(P<0.05)。结论:SFN能降低膀胱癌细胞的侵袭能力,其作用机制与抑制膀胱癌细胞EMT过程有关。Objective： To investigate the effects of sulforaphane on the invasion and Epithelial-to-Mesenchymal Transition（EMT） in human bladder cancer cell and its mechanism in vitro.Methods： The migration and invasion of sulforaphane on T24 cells were tested by transwell assay.Western blot analysis was used to investigate the protein expression of E-cadherin and vimentin.Results： Sulforaphane（10μmol/L） could dramatically enhance the expression of E-cadherin,and meanwhile reduce the expression of vimentin in T24 cells（P〈0.05）.Conclusion： These results suggest that sulforaphane can obviously suppress the invasion of T24 cell.Its mechanism is associated with the Epithelia-to-Mesenchymal Transition.

关 键 词：莱菔硫烷 膀胱癌 侵袭 EMT 

分 类 号：R737.14[医药卫生—肿瘤] R285.5[医药卫生—临床医学]