Rab23在鳞癌细胞系中的表达及其对鳞癌细胞侵袭的影响  

The Expression of Rab23 in Squamous Cell Carcinoma Cell Lines and Its Role on the Cell Invasion of SCC

作　　者：唐利 刘博强 简强 廖文俊 迟素敏[2] 李承新 

机构地区：[1]第四军医大学第一附属医院全军皮肤病研究所,陕西西安710032 [2]第四军医大学生理学教研室,陕西西安710032

出　　处：《现代生物医学进展》2012年第13期2440-2443,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30872264)

摘　　要：目的:观察Rab23在鳞癌细胞系中的表达及其对鳞癌细胞侵袭的影响。方法:western blot检测Rab23在四个头颈部鳞癌细胞系中的表达,对鳞癌细胞系HSQ-89用脂质体转染小干扰RNA,对Sa3用脂质体转染Rab23质粒,分别干涉或过表达Rab23,用铺有基质胶的transwell小室检测细胞侵袭能力,细胞划痕试验检测迁移能力。结果:Rab23在四个鳞癌细胞系中均有表达,在Hacat中不表达,HSQ-89细胞经Rab23干涉后细胞侵袭能力减弱,而Sa-3细胞过表达Rab23后细胞侵袭能力增强。干涉或过表达Rab23后,鳞癌细胞的迁移能力无差异。结论:Rab23可促进鳞癌细胞侵袭。Objective： To observe the expression of Rab23 in Squamous Cell Carcinoma（SCC） cell lines and its role on the cell invasion of SCC.Methods： The expression of Rab23 in four SCC cell lines was detected by western blot.RAB23 siRNAs or RAB23 cDNA were transfected by Oligofectamine in Hsq-89 cells or Sa-3 cells respectively.Biocoat Matrigel invasion chambers were used to detect the invasion ability.Result： Rab23 expression was found in all SCC cell lines except for Hacat.Cellular invasion was significantly reduced by siRNA silencing of RAB23 in Hsq-89 cells,whereas significantly enhanced by overexpression of RAB23 in Sa-3 cells.Silencing or overexpression of Rab23 had no effect on SCC cell migration.Conclusion： Rab23 can enhance the invasion of SCC cells.

关 键 词：Rab23 鳞癌 侵袭 

分 类 号：R739.5[医药卫生—肿瘤]