人脑胶质瘤细胞中GDNF基因启动子1区甲基化状态的研究  

Study of the Methylation Status of Promoter 1 Region of GDNF Gene in Human Glioma Cells

作　　者：任庆先[1] 王建村[2] 王莉[3] 高殿帅[4] 虞正权[1] 

机构地区：[1]苏州大学附属第一医院神经外科,江苏苏州215006 [2]徐州医学院附属医院神经外科 [3]徐州医学院附属医院神经内科 [4]徐州医学院神经生物实验室,江苏徐州221002

出　　处：《现代生物医学进展》2012年第15期2819-2821,2803,共4页Progress in Modern Biomedicine

基　　金：江苏省高校自然科学基金(11KJB180012);青年科学基金项目(81101899)

摘　　要：目的:观察GDNF启动子1区在人脑胶质瘤细胞中的甲基化修饰状态,以期探讨其对于GDNF在胶质瘤中高表达的影响。方法:基因测序检测10例胶质瘤与5例正常脑组织中GDNF基因序列,比较其基因是否有突变发生;重亚硫酸盐修饰后基因测序检测20例胶质瘤(10例低级别和10例高级别)与5例正常脑组织中GDNF启动子1区甲基化修饰状态。结果:GDNF启动子1区基因在胶质瘤中没有发生突变;GDNF启动子1区甲基化修饰在正常脑组织、低级别、高级别中发生率分别为72.25%、86.25%、86.75%。在胶质瘤中的甲基化修饰水平比正常脑组织明显增高(P<0.05),而高低级别之间无显著性差异。结论:在胶质瘤细胞中,GDNF启动子1区发生了高甲基化修饰,这种修饰很可能会影响GDNF基因的表达。Objective： To observe the methylation status of GDNF promoter 1 region in human glioma,in order to explore the effect of methylation on the expression of GDNF in glioma.Methods： Genesequencing detected the GDNF gene order in 10 cases of glioma and 5 normal brain tissues,to compare whether their genetic mutations have occurred;the methylation modified status of GDNF promoter 1 region in 20 cases of glioma（10 cases of low-level and 10 cases of high-level） and 5 cases of normal brain tissues were detected through genesequencing after bisulfite modification.Results： The mutations of GDNF gene in promoter 1 region were not observed in glioma;the methylation of GDNF gene in promoter 1 region in normal brain tissues,low-level and high-level glioma were 72.25%,86.25%,86.75% respectively.The methylation level in glioma was significantly higher when compared to normal brain tissues（P〈0.05）;while there was no significant differences in high-and low-level tissues.Conclusions： Hypermethylation occuerred in GDNF promoter 1 region might influence the expression of GDNF in glioma cell.

关 键 词：胶质瘤细胞 角质细胞源性神经营养因子 启动子 甲基化 

分 类 号：R739.41[医药卫生—肿瘤]