白矾保肝降脂方对非酒精性脂肪肝大鼠肝组织ADP、TNF-α的影响(英文)  

Effects of Alum Hepatoprotective on NAFLD Rat's Hepatic Tissue ADP and TNF-α

作　　者：支晓丽[1] 王者令 刘中景[2] 孙旭成[1] 李晓丹[1] 宋霆[1] 

机构地区：[1]青岛大学医学院,山东青岛266021 [2]青岛市传染病医院,山东青岛266033

出　　处：《现代生物医学进展》2012年第15期2855-2858,共4页Progress in Modern Biomedicine

摘　　要：目的:观察白矾保肝降脂方对非酒精性脂肪肝大鼠肝组织脂联素(ADP)、肿瘤坏死因子α(TNF-α)的影响。方法:48只健康雄性Wistar大鼠随机分为4组,每组12只。高脂高糖饲料喂养制备大鼠非酒精性脂肪肝模型。8w病理显示造模成功后,每组给予相应干预,连续4w。酶联免疫吸附(ELISA)法测定各组大鼠肝组织中ADP、TNF-α浓度,HE染色观察肝脏病理变化。结果:与正常对照组大鼠比较,模型对照组肝组织中ADP浓度明显降低(P<0.05),TNF-α明显升高(P<0.05);与模型对照组大鼠比较,白矾保肝降脂方组与水林佳组肝组织中ADP浓度均明显升高(P<0.05),TNF-α明显降低(P<0.05);白矾保肝降脂方组大鼠较水林佳组,肝组织中ADP浓度显著升高(P<0.05),TNF-α无显著性差异(P>0.05)。结论:白矾保肝降脂方能显著升高肝组织中ADP浓度,降低TNF-α,明显改善肝细胞脂肪变性,缓解炎性浸润,可能是其治疗非酒精性脂肪肝的作用机制之一。Objective： To investigate the effects of alum hepatoprotective on adiponectin（ADP） and tumor necrosis factor（TNF-α） in hepatic tissue of the non-alcoholic fatty liver disease（NAFLD） rat.Methods： Forty-eight healthy male Wistar rats were chosen and randomly divided into four groups,with 12 rats in each group.The high fat and high glucose diet was fed to make NAFLD rat models.When the modeling was accomplished after eight weeks,each group would be intervened respectively for four weeks.Then enzyme-linked immunosorbent assay（ELISA） was applied to detect ADP and TNF-α.HE mucus stain was used to observe liver pathologic changes.Results： Compared with that in the normal group,the ADP density in the hepatic tissue of model group decreased obviously（P0.05） and the TNF-α density increased（P0.05）.Compared with that in the model group,the ADP density in the group which was treated with alum hepatoprotective（alum hepatoprotective group） increased remarkably（P0.05） and the TNF-α density decreased（P0.05）;Meanwhile,the ADP density in Silybinin group increased obviously（P0.05） and TNF-decreased（P0.05）.Compared with that of the Silybinin group,ADP density of the alum hepatoprotective group increased distinctly（P0.05） and TNF-α had no significant difference（P0.05）.Conclusion： Alum hepatoprotective can significantly increase the ADP density and decrease TNF-α in hepatic tissue,greatly improve hepatic macrovesicular steatosis and reduce inflammatory infiltration,which is probably one action mechanism to treat NAFLD.

关 键 词：白矾保肝降脂方 非酒精性脂肪肝 大鼠 ADP TNF-Α 

分 类 号：Q95-03[生物学—动物学] R575.5[医药卫生—消化系统]