高能X线和人参皂甙Rb_1对人脑胶质瘤细胞SHG-44的影响  

Effects of High Energy X-ray and Ginsenoside Rb_1 on Human Glioma SHG-44 Cell

作　　者：问静[1] 何侠[1] 赵洪瑜[2] 仇晓军[2] 王燕[2] 陈晓珏[2] 季斌[2] 

机构地区：[1]江苏省肿瘤医院,江苏南京210009 [2]南通大学附属医院,江苏南通226001

出　　处：《肿瘤学杂志》2012年第4期266-269,共4页Journal of Chinese Oncology

摘　　要：[目的]探讨高能X线和人参皂甙Rb1对人脑胶质瘤细胞株SHG-44的影响。[方法]应用MTT法检测细胞增殖情况;流式细胞仪检测细胞周期的变化;RT-PCR法检测MMP-2、MMP-9mRNA的表达水平。[结果]高能X线照射后SHG-44细胞增殖受到抑制,且随照射剂量加大抑制作用增强。当加入人参皂甙Rb1后,细胞增殖抑制更加明显;高能X线照射后SHG-44细胞表现为G2/M期阻滞,而人参皂甙Rb1可缓解G2/M期阻滞现象;辐射促进MMP-2、MMP-9的表达,且随照射剂量的增加表达量增加,而人参皂甙Rb1抑制其表达,与药物浓度呈反比。[结论]人参皂甙Rb1与高能X线协同抑制SHG-44的增殖,辐射后肿瘤细胞侵袭能力增强,而人参皂甙Rb1使照射后肿瘤细胞侵袭能力下降。[Purpose] To investigate the effects of high energy X-ray and ginsenoside Rb1 on human glioma SHG-44 cell line.[Methods] MTT method was applied to detect the cell proliferation conditions.Flow cytometry was applied to observe the change of cell cycle.The expressions of MMP-2 and MMP-9 mRNA were detected by reverse transcription polymerase chain reaction（RT-PCR）.[Results] The SHG-44 cell proliferation was inhibited after 12h of radiation,which boosted with the increasing of radiation dose.The inhibition of proliferation was more obvious when adding ginsenoside Rb1.The SHG-44 cell showed that the G2/M phase arrested after radiation,and the delay of G2/M phase alleviated when adding ginsenoside Rb1.The expressions of MMP-2 and MMP-9 in SHG-44 cell increased along with the increasing of X-ray dose.When ginsenoside Rb1 was added to the cell culture,the expressions of MMP-2 and MMP-9 were inhibited,which decreased along with increasing of the dose of ginsenoside Rb1.[Conclusion] Ginsenoside Rb1 and high energy X-ray radiation show synergistic inhibition of cell proliferation.After radiation,the ability of tumor invasion and metastasis increases,while ginsenoside Rb1 can decrease the ability of invasion and metastasis by the down-regu-lation of MMPs mRNA expression.

关 键 词：高能X线 人参皂甙RB1 胶质细胞瘤 基质金属蛋白酶 

分 类 号：R739.41[医药卫生—肿瘤]