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机构地区:[1]河南师范大学生命科学学院,河南新乡453007
出 处:《河南师范大学学报(自然科学版)》2012年第3期141-144,共4页Journal of Henan Normal University(Natural Science Edition)
基 金:河南省科技攻关项目(49604004)
摘 要:在相等土壤肥力和相同体积花盆中培养的大豆幼苗,分别用浓度5、10、20mmol.L-1的抗坏血酸(VC)和自来水(CK)浇灌,18d后逐步测定正在扩张生长期的叶片中可溶性糖、可溶性蛋白含量、硝酸还原酶(NRA)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性.结果表明:10mmol.L-1 VC处理的大豆叶片可溶性糖、可溶性蛋白含量,SOD酶活性比CK均有所提高;3个处理的硝酸还原酶(NRA)活性均比CK有所增大;10mmol.L-1 VC处理对POD酶活性影响不明显.Soybean [Glycine max (L.)Merr. ] seedlings have been cultured in flowerpots with equal size and the same soil. In this pot experiment, soybean seedlings are poured with 5 mmol/L, 10 mmol/L, 20 mmol/L ascorbic acid(VC) and tap water(CK), 18 days later, the soluble sugar content and the soluble protein content together with the activity of nitrate reductase(NRA), the activity of superoxide dismutase(SOD) and the activity of peroxidase(POD) in leaves are measured. The results show that the content of soluble sugar and soluble protein and the activity of superoxide dismutase(SOD) in the soybean leaves which are poured with 10mmol/L VC have been enhanced contrasted with CK~ the activity of nitrate reductase(NRA) in the leaves which are poured with the three different consistence of ascorbic acid(VC) are higher than CK; and 10mmol/LVC have no obvious effect on the activity of peroxidase(POD).
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