香菇多糖测定的方法学研究  

The Method Research of Lentinan Determination

作　　者：于村[1] 丁钢强[1] 俞莎[1] 张双凤[1] 吕鑫 

机构地区：[1]浙江省卫生防疫站,杭州310009 [2]浙江大学公卫学院

出　　处：《中国公共卫生》2000年第3期245-246,共2页Chinese Journal of Public Health

基　　金：浙江省卫生厅科研基金课题

摘　　要：为选出一种比较准确、方便的香菇多糖的测定方法 ,对蒽酮—硫酸法、苯酚—硫酸法、直接滴定法三法进行了比较。实验结果表明 :蒽酮—硫酸法所测香菇多糖含量偏高 ,回收率过高 (113 6 %～ 145 1% ) ;直接滴定法灵敏度不高 ,回收率偏低 (42 8%～ 85 8% ) ;而苯酚—硫酸法不仅灵敏度高 ,回收试验也较理想 (92 5 %～ 110 2 % )。因而 。In order to select an accurate,simple method of lantinan determination,this article compare the different methods of anthrone sulfuric method,phenolsulfuric method and direct titration method.The result showed:lentinan detected by anthrone sulfuric method is higher than other methods,the restore rate is 113 6%～145%.The sensitivity is not good of direct titration method,the restore rate is only 42 8%～85 8%.Phenol sulfuric method is not only higher in sensitivity,but also is satisfied of restore rate(92 5%～110).The result declares that phenol sulfuric method is a better method in detecting the lentinan.

关 键 词：香菇多糖 蒽酮-硫酸法 苯酚-硫酸法 测定 

分 类 号：R284.1[医药卫生—中药学]