体外培养人涎腺多形性腺瘤细胞及表面标记物的初步研究  

Characteristics of Human Salivary Pleomorphic Adenoma Cell Cultured in Vitro and the Surface Markers

作　　者：孟超[1,2] 高岩[3] 王衣祥[1,2] 李盛林[1,2] 

机构地区：[1]北京大学口腔医学院口腔医院中心实验室,北京100081 [2]北京大学口腔医学院口腔医院口腔颌面外科,北京100081 [3]北京大学口腔医学院口腔医院口腔病理科,北京100081

出　　处：《科学技术与工程》2012年第17期4099-4102,4107,共5页Science Technology and Engineering

基　　金：国家自然科学基金(39470754);教育部985项目(985-2-040-115)资助

摘　　要：体外培养人涎腺多形性腺瘤细胞,观察细胞生长及形态特征,探讨体外培养的细胞与原发肿瘤组织的表面标志物表达情况。取15例原发多形性腺瘤进行体外培养,运用免疫组化法检测表面标志物CK5/6、CK7、CK8/18、CK14、CK20、α-SMA、Caplonin、P63及vimentin及肌上皮表面标记物α-SMA、calponin及p63在细胞和肿瘤组织中的表达。结果发现体外培养的多形性腺瘤细胞呈多样形,可同时表达角蛋白、肌上皮表面标记物及波形蛋白。提示体外培养的多形性腺瘤细胞可能大部分来源于肌上皮细胞及导管上皮细胞等,由闰管细胞或者闰管储备细胞分化而来,这为研究涎腺多形性腺瘤肿瘤生物学多样性提供了实验依据。To study the biological characteristics of salivary gland pleomorphic adenoma tumor.Fifteen tumor tissue derived from salivary gland pleomorphic adenoma were cultured in vitro.Immunohistochemical staining was used to investigate the expression of surface markers such as CK5/6,CK7,CK8/18,CK14,CK20,p63,α-SMA,calponin,vimentin.The results showed that the cultured human salivary pleomorphic adenoma cells were varied and expressed both keratin and myoepithelial cell surface markers which indicated that the cells may be from myoepithelial cells and ductal epithelial cells.The results provide experimental basis for the study of the multiple biological characteristics of salivary gland pleomorphic adenoma tumor.

关 键 词：多形性腺瘤 肌上皮细胞 复发 

分 类 号：R730.261[医药卫生—肿瘤]