放射配基结合分析法对不同细胞uPAR表达的比较研究  

Studies on the Comparison of Expression of Urokinase -- Type Plasminogen Activator Receptor on Cell Surface Between Five Cultured Human Tumor Cell Lines and Five Kinds of Normal Cells

作　　者：张启良[1] 陈岚[1] 刘玮[1] 张健[1] 杨君蕊[1] 张建平[1] 

机构地区：[1]上海第二医科大学病理生理学教研室

出　　处：《上海第二医科大学学报》2000年第2期102-104,178,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金!(39670327);上海市科技发展基金!(97ZB14018);胡应洲基金

摘　　要：目的测定和比较不同正常细胞及人类肿瘤细胞膜上尿型纤溶酶原活化素受体（ｕＰＡＲ）的表达，了解肿瘤细胞表达ｕＰＡＲ的基本性质。方法建立并应用受体放射配基结合分析法（ｒｅｃｅｐｔｏｒ－ ｒａｄｉｏ－ ｌｉｇａｎｄ ｂｉｎｄｉｎｇ ａｓｓａｙ， ＲＢＡ）测定细胞膜上的ｕＰＡＲ。结果　成纤维细胞经不同处理，Ｕ９３７细胞以ＰＭＡ处理不同时间，测定ｕＰＡＲ的结果无论表达量或平衡解离常数（Ｋｄ）的变化均与文献一致。对正常细胞和肿瘤细胞株共１０种测定膜上ｕＰＡＲ，结果肺癌、肝癌等５种人类肿瘤细胞比人成纤维细胞、牛内皮细胞等正常组织细胞的表达数明显多。结论　本研究进一步证明了ｕＰＡＲ表达增强是多种恶性肿瘤细胞的一种特点。Objective This study was designed to quantitatively determine and compare the expression of urokinase -- type plasminogen activator receptor (uPAR) in different normal cells and/with different cultured tumor cell lines. Methods The method of radioligand binding assay of receptor (RBA) for examination of uPAR on the cell membrane has been established with 125I -- DFP -- uPA, a radioligand agent prepared in our laboratory, and used to monitor the uPAR levels in all experiments. Results The number of uPAR expressed and the affinity for uPAR to combine with its specific lgand, uPA, were examined using RBA method in ten buds of cells, including five kinds of normal cells and five cultured tumor cell lines, such as myeloid leukemic cell lines of U937 and HL-- 60, lung cancer cell line A410, hepatic cancer cell line Bel 7402 and gastric carcinoma cell line N -- 45. The uPAR expression was not found in human red blood cells, and the uPAR expression of all examined malignant tumor cell lines was much stronger than that of the normal tissue cells. Conclusion it is further confirmed that high uPAR expression is characteristic for most of the malignant tumors' cells. The method can be used to study the role of uPAR and the influence factors for uPAR expression in laboratory experiments.

关 键 词：肿瘤 放射配基结合 UPAR 表达 

分 类 号：R730.23[医药卫生—肿瘤]