丹参在兔眼小梁切除术后抗增生作用的研究  

Antiproliferative effect of salvia miltiorrhiza monomer IH764-3 on trabeculectomy of rabbit eyes

作　　者：孙海燕[1] 刘建宗[2] 赵平[1] 

机构地区：[1]河北医科大学第三医院眼科,石家庄050000 [2]河北医科大学第二医院眼科

出　　处：《中华眼外伤职业眼病杂志》2012年第6期401-405,共5页Chinese Journal of Ocular Trauma and Occupational Eye Disease

摘　　要：目的探讨丹参单体（IH764—3）在兔眼小梁切除术后的抗增生作用。方法将32只新西兰大耳白兔随机分为4组，每组8只：空白对照组、阳性对照组、实验I组、实验Ⅱ组，双眼均行标准小梁切除术，实验I组、Ⅱ组分别于术后结膜下注射2g·L。或5g·L。丹参单体0．2ml，术后第1周1次／d，第2周1次／2d，共10次。空白对照组和阳性对照组同样注射等量生理盐水和25g·L-1的5．氟脲嘧啶（5-FU）。术前及术后3d、7d、15d、30d各组观察眼压、滤过泡、滤过道开放情况及瘢痕形成的组织结构、增生细胞核抗原阳性细胞计数等。结果观察术后7d、15d、30d功能性滤过泡数、眼压控制情况、增生细胞核抗原（PCNA）阳性细胞等，实验Ⅱ组疗效均优于其他3组。且未发现结膜伤口漏或角膜上皮细胞损伤等副作用。结论丹参单体在小梁切除术中有抑制成纤维细胞增生的作用。丹参单体应用于小梁切除术中及术后，其抗增生能力较5-Fu强，而副作用小，并发症少。Objective To explore the antiproliferative effect of salvia miltiorrhiza monomer IH764-3 on trabeculectomy of rabbit eyes. Methods Thirty-two rabbit eyes were divided into 4 groups randomly with eight rabbits in each group： control group, positive control group, experiment group I and experiment group II Both eyes of each rabbit underwent standard trabeculectomy. Subconjunctival injections of 0.2 mL 2 g/L IH764-3, 5 g/L IH764-3, normal saline solution and 25 g/L 5-FU were performed in experiment group I , group lI, control group and positive control group once per clay in the first week and once every two days in the second week after surgery. Observation items included preoperative intraocular pressure （IOP） , postoperative lOP, filtering bleb, filtering passway, scar structure and proliferating cell nuclear an- tigen （PCNA）. Results Experiment group ]] had better therapeutic effect than other three groups on func- tional filtering bleb, IOP and PCNA at 7 d, 15 d and 30 d postoperatively. Conjunctival wound leaking or corneal epithelial damage had not been found. Conclusion IH764-3 has stronger antiproliferative effect on trabeculectomy than 5-FU with less intraoperative and postoperative side effects.

关 键 词：小梁切除术 丹参单体IH764-3 纤维增生 青光眼 

分 类 号：R779.6[医药卫生—眼科]