气相色谱-质谱法定性分析麝香保心丸挥发性入血成分的研究  

Identification of volatility components of Shexiang Baoxin pill in rat plasma by GC-MS

作　　者：郭黎安[1] 王淑萍[2] 向丽[2] 姜鹏[2] 张卫东[2] 柳润辉[2] 

机构地区：[1]第二军医大学训练部,上海200433 [2]第二军医大学药学院天然药物化学教研室,上海200433

出　　处：《药学实践杂志》2012年第3期207-210,共4页Journal of Pharmaceutical Practice

摘　　要：目的建立气相色谱-质谱法(GC-MS)定性分析麝香保心丸挥发性入血成分的方法。方法健康雄性SD大鼠给予麝香保心丸混悬液灌服(剂量3.4 g/kg体重),于口服30 min后肝门静脉取血,蛋白沉淀法处理血浆样品进行GC-MS分析。分析条件:Thermo TR-5 ms色谱柱;Xcalibur色谱工作站;载气为氦气;载气流速为1.0 ml/min;进样口温度为220℃;离子源温度为250℃;梯度升温;进样体积为1μl;分析时间59 min。结果通过比较麝香保心丸提取液、空白组及给药组,及与在线NIST05数据库比对,共识别和鉴定10个化学成分,其中6个为麝香保心丸中原型成分,其余4个为代谢物。结论本研究建立了一种快速筛选麝香保心丸中挥发性入血成分的方法,可为其深入研究提供基础。Objective To identify the volatile components in rat serum of Shexiang Baoxin Pill （SBP） by gas chromatography couples with mass spectrometry （GC-MS）. Methods Serum samples from control and SBP administration rats were deproteinated by adding methanol prior to gas chromatography. The separation was carried out on a Thermo TR-5 ms column （30 m × 0. 25 mm I. D. ）. The inlet temperature was set at 220 ℃ and ion source temperature was 250 ℃ with gradient warming. The carrier gas was N2 with a gas flow rate of 1.0 ml/min, and the injection volume was 1ul. The total analysis time was 59min. The data acquisition were performed on Xcalibur workstation. Results Ten components including 6 components from SBP and 4 metabolites were observed from a comprehensive analysis of the chromatography of SBP solution. All the observed components were identified by using the online NIST05 library. Conclusion An integrated method for screening volatile bioactive components in serum after oral administration of SBP was established, which provided helpful information for further mechanism research.

关 键 词：麝香保心丸 血清药物化学 冠心病 气相色谱-质谱联用 

分 类 号：R541.4[医药卫生—心血管疾病]