响应面法优化P.acidipropionici产酸发酵培养基  

Optimization of fermentation medium of propionic acid production by Propionibacterium acidipropionici using response surface methodology

作　　者：刘寅[1] 和晶亮[2] 赵继红[1] 魏明宝[1] 杨雪鹏[1] 魏东芝[1] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002 [2]河南工程学院,河南郑州451191

出　　处：《中国酿造》2012年第5期59-63,共5页China Brewing

基　　金：河南省重大公益项目(101100910300);河南省科技创新人才项目(094200510006);郑州轻工业学院博士科研基金(2010BSJJ019)

摘　　要：利用响应面分析法优化产酸丙酸杆菌发酵产酸培养基。在单因素试验的基础上,采用Box-Behnken试验设计,选定甘油、混合氮源(酵母提取物:胰酶大豆肉汤=2:1)和磷酸氢二钾3个关键因子为响应因子,以丙酸产量为响应值建立多元二次回归方程,在分析各个因素的显著性和交互作用后,确定了产酸丙酸杆菌发酵产酸的最优培养基为:甘油44.4g/L、混合氮源(酵母提取物:胰酶大豆肉汤=2∶1)27.0g/L、K2HPO42.0g/L,丙酸产量最大预测值为20.75g/L。经过优化,丙酸产量提高了151%,实验值与预测值基本相符。Fermentation medium for propionic acid using Propionibacterium acidipropionici CGMCC1.2225 was optimized by response surface analysis（RSA）.Based on single factor experiment,three critical factors（the content of glycerol,mixed nitrogen sources and K2HPO4） were selected as response factors and the propionic acid yield was used as response value to establish the quadric regression equation.The optimum fermentation medium was as followed：glycerol 44.4g/L,mixed nitrogen sources（yeast extract∶tryptic soy broth=2∶1） 27.0g/L and K2HPO4 2.0g/L.Under these conditions,the highest yield of propionic acid was predicted to be 20.75g/L.After optimization,the propionic acid yield was increased by 151%.The experimental values were basically fit with the predicted values.

关 键 词：发酵培养基优化 产酸丙酸杆菌 丙酸 BOX-BEHNKEN设计 响应面法 

分 类 号：Q93-335[生物学—微生物学]