X线交叉互补修复基因1多态性与肺癌的相关性研究  

The relationship between lung cancer and genetic polymorphism of XRCC1

作　　者：都勇[1] 储德节[1] 胡志雄[1] 史继敏[2] 范卫[2] 

机构地区：[1]复旦大学附属金山医院呼吸内科,上海200540 [2]复旦大学附属金山医院中心实验室,上海200540

出　　处：《临床内科杂志》2012年第5期346-348,共3页Journal of Clinical Internal Medicine

摘　　要：目的探讨X线交叉互补修复基因1（XRCC1）多态性与肺癌之间的关系，研究吸烟与易感基因的交互作用。方法采用病例对照研究，应用聚合酶链反应一限制性片段长度多态性（PCR—RFLP）技术，检测XRCC1—194、XRCC1—399基因型频率。结果XRCC1—194突变型基因频率在肺癌组及时照组中分别为30．5％和20％，两者比较差异有统计学意义（P〈0．05）；XRCC1-399突变型基因频率在两组中分别为20．5％和15％，两者比较差异无统计学意义（P〉0．05）；但XRCC1—194突变型基因频率在小细胞肺癌患者及非小细胞肺癌患者中分别为18．18％和33．97％，两者比较差异有统计学意义（P〈0．05）。有吸烟史而无XRCC1—194单核苷酸多态性时的相对危险度为1．851，尤吸烟史而有XRCC1—194单核苷酸多态性时的相对危险度为2．336，有吸烟史且XRCC1-194单核苷酸多态性均存在时的相对危险度为8．01。结论XRCC1—194基因多态性与肺癌的发生相关，主要与非小细胞肺癌的发生密切相关；吸烟史及XRCC1—194单核苷酸多态性在肺癌的发生中有交互作用。Objective To investigate the relationship between lung cancer and gene polymorphism of XRCC1 as well as to research the interactive effects between smoking and the susceptible gene. Methods We use a case-control study with a polymerase chain reaction-fragment polymorphism（ PCR- RFLP） method to detect the frequency of XRCC1-194,XRCC1-399 genetypes. Results The heterogenesis gone frequency of XRCC1-194 in lung cancer group and healthy control group were 30.5% and 20% , which difference was significant（ P 〈 0.05 ）. the heterogenesis gene frequency of XRCC1-399 were 20.5% and 15% , which difference was not significant （ P 〉 0. 05 ） ; but the heterogenesis gene frequency of XRCCl-194 in small cell lung cancer group and non - small cell lung cancer group were 18. 18% and 33.97% ,which difference was significant（P 〈 0.05） ;Except XRCC1-194 SNP,the relative risk of smok- ing factor was 1.851, except smoking factor, the relative risk of XRCCl-194 SNP was 2. 336, the relative risk of smoking factor and XRCC1-194 SNP was 8.01. Conclusion Polymorphism of XRCC1-194 have relations with the development of lung cancer, especially with the non-small cell lung cancer;there is an interaction between smoking factor and XRCC1-194 SNP.

关 键 词：肺癌 X线交叉互补修复基因1 单核苷酸多态性 

分 类 号：R734.2[医药卫生—肿瘤]