凝胶净化/超高效液相色谱串联质谱法测定调味酱中32种工业染料  

Determination of 32 Industrial Dyes in Seasoning Paste by Gel Permeation Chromatography and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：郭新东 冼燕萍 罗海英 黄金凤 蔡玮红 吴玉銮 

机构地区：[1]广州市质量监督检测研究院国家加工食品质量监督检验中心(广州),广东广州510110

出　　处：《分析测试学报》2012年第6期658-663,共6页Journal of Instrumental Analysis

基　　金：广东省科技计划项目(2007B023001001);广州科信局项目(11C13190775)

摘　　要：建立了调味酱中32种工业染料的超高效液相色谱串联质谱分析方法。样品用乙腈均质提取,经凝胶色谱净化,浓缩后,以甲醇定容,超高效液相色谱分离,串联四极杆质谱多反应监测方式监测。结果表明,32种工业染料在各自的线性范围内线性关系良好,相关系数(r)均大于0.994,定量下限为0.2～5.0μg/kg,加标回收率为53%～115%,相对标准偏差为3.9%～22.4%。该方法具有前处理简单、净化效果好、灵敏度高的优点,适用于调味酱中多组分工业染料的快速确认和定量检测。A method for the analysis of 32 industrial dyes in seasoning paste was developed by ultra performance liquid chromatographytandem mass spectrometry（ UPLC -MS/MS） combined with gel permeation chromatography（GPC） purification. The homogenized sample was extracted with acetonitrile, and then purified by GPC. The targeted compounds were detected by MS/MS system with eletrospray ionization（ESI） under multiple reaction monitoring（MRM） mode. The result indicated that the limits of quantitation（LOQs） of 32 dyes were in the range of 0.2 -5 μg/kg. The linear correlation coefficients of 32 dyes were more than 0. 994. The average recoveries ranged from 53% to 115% with RSDs of 3.9% -22.4%. This method was suitable for the identification and quantification of multi-component dyes in seasoning paste due to its simplicity, good purification and high sensitivity.

关 键 词：调味酱 工业染料 凝胶净化 超高效液相色谱-电喷雾电离串联质谱 

分 类 号：O657.63[理学—分析化学] TS529.3[理学—化学]