脂肪酶修饰丝网印刷电极测定三油酸甘油酯  

Using Lipase Modified Screen-printed Electrode as Glyceryltrioleate Biosensor

作　　者：刘韩凤[1] 郭春景[1] 叶永康[1] 罗水忠[1] 姜绍通[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《分析测试学报》2012年第6期680-685,共6页Journal of Instrumental Analysis

基　　金：国家863计划资助(2010AA101503)

摘　　要：制备了用于快速检测三油酸甘油酯的脂肪酶修饰丝网印刷电极(SPE)电化学传感器单元。用循环伏安法(CV)和计时电流法(CC)研究了三油酸甘油酯在脂肪酶修饰SPE上的电化学行为,确定了测定甘油酯的最优条件,建立了测定三油酸甘油酯浓度的电化学方法。其中CV法的线性范围为0.01～5 g.L-1,检出限为2.5 mg.L-1;CC法的线性范围为0.001～5.50 g.L-1,检出限为0.2 mg.L-1。以CV法考察样品的加标回收率,6个葵花籽油样品的加标回收率为94%～105%。A rapid method for the determination of glyceryltrioleate was developed by using screenprinted electrode （SPE） modified with lipase as an electrochemical biosensor. Cyclic voltammetry （CV） and chronocoulometry（CC） were applied to characterize the behavior of glyceryhrioleate on the surface of the lipase-modified SPE. The optimal conditions of glyceryltrioleate determination were investigated. A linear response to glyceryltrioleate in the concentration ranges of 0. 01 - 5 g · L- 1 with a detection limit of 2. 5 mg · L- 1 for CV, and 0. 001 - 5.50 g · L- 1 with a detection limit of 0.2 mg · L- 1 for CC were obtained. The method was used in the determination of 6 sunflower seed oil samples with spiked recoveries of 94% -105%.

关 键 词：丝网印刷电极 脂肪酶 三油酸甘油酯 

分 类 号：O657.1[理学—分析化学] TQ630.41[理学—化学]