人参皂苷Rg3调控WNT/β-catenin信号通路抑制结肠癌细胞生长的实验研究  

Effect of Ginsenoside Rg3 for Wnt/β-catenin Signaling Pathway of Colon Carcinoma Cells

作　　者：何运元[1] 王营[1] 王黎[2] 赵琳琳[1] 徐学军[2] 

机构地区：[1]徐州医学院,江苏徐州271000 [2]郑州大学基础医学院,河南郑州450052

出　　处：《肿瘤基础与临床》2012年第3期189-194,共6页journal of basic and clinical oncology

摘　　要：目的探讨人参皂苷Rg3通过影响WNT/β-catenin信号通路中关键蛋白β-catenin从而阻断结肠癌细胞生长机制的研究。方法 MTT法检测不同浓度人参皂苷Rg3对人结肠癌细胞系SW480、HCT116细胞增殖的影响;流式细胞仪检测不同浓度人参皂苷Rg3对SW480、HCT116细胞凋亡及磷酸化β-catenin的影响;RT-PCR和Western blot法检测HCT116细胞中β-catenin及c-myc的表达。结果人参皂苷Rg3具有抑制SW480、HCT116细胞增殖和促进凋亡的能力。一定浓度人参皂苷Rg3能够降低β-catenin蛋白的磷酸化程度,下调β-catenin mRNA的表达,下调β-cetanin和c-myc蛋白的表达。结论人参皂苷Rg3具有一定的抗肿瘤活性,可有效抑制HCT116和SW480细胞生长,且这种作用可能是通过下调β-catenin磷酸化来实现的。Objective To investigate the mechanisms of ginsenoside Rg3 inhibiting the growth of colon carcinoma cells through affecting the Wntβ-catenin signaling pathway. Methods MTr assay was used to examine the effect of ginsenoside Rg3 for the cell proliferation of SW480, HCT116 cells; flow eytometry was used to examine the effect of ginsenoside Rg3 for the cell apoptosis of SWdS0, HCT116 cells and the 13-catenin phosphorylate ; RT-PCR and Western blot method were used to examine the expression of β-catenin and c-myc. Results Ginsenoside Rg3 could inhibit the cell proliferation of SW480, HT116 cells and promote their cell apoptosis. Ginsenoside Rg3 could decrease the β-catenin phosphorylate, downregulated the mRNA expressions of β-catenin and the protein expressions of β-catenin and c-myc. Conclusion Ginsenoside Rg3 has the anticancer activity, can inhibit the growths of colon carcinoma cells , and the effect may be related the downregulation of β-catenin phosphorylate.

关 键 词：人参皂苷RG3 WNT/Β-CATENIN信号通路 Β-CATENIN 磷酸化 结肠癌 

分 类 号：R735.3[医药卫生—肿瘤]