Ni和Cu胁迫对骆驼蓬抗氧化酶活性的影响  

Effects of Ni and Cu on antioxidative enzymes in Peganum harmala

作　　者：鲁艳[1,2] 李新荣[3] 何明珠[3] 冯丽[3] 黄磊[3] 曾凡江[1,2] 

机构地区：[1]中国科学院新疆生态与地理研究所,新疆乌鲁木齐830011 [2]新疆策勒荒漠草地生态系统国家野外科学观测研究站,新疆策勒848300 [3]中国科学院寒区旱区环境与工程研究所沙坡头沙漠研究试验站,甘肃兰州730000

出　　处：《草业学报》2012年第3期147-155,共9页Acta Prataculturae Sinica

基　　金：新疆科技重大专项(200733144-2);国家杰出青年科学基金(40825001);国家科技支撑计划项目(2009BAC54B01)资助

摘　　要：采用温室盆栽法,研究Ni、Cu单一处理[0(对照),50,100,200,400mg/kg]对金昌Ni/Cu尾矿库先锋植物骆驼蓬幼苗Ni、Cu积累、膜脂过氧化程度与抗氧化酶系统的影响。结果表明,骆驼蓬幼苗叶片及根部Ni、Cu含量随土壤介质中Ni、Cu含量的增加而增加,Ni主要富集在骆驼蓬的叶片,而Cu主要富集在骆驼蓬的根部;叶片及根部丙二醛(MDA)含量总体随Ni、Cu处理浓度升高而增加,膜脂过氧化程度加剧;Ni胁迫下,骆驼蓬叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸酶(APX)和谷胱甘肽还原酶(GR)活性较对照均有所增强,共同组成植物体内一个有效的活性氧自由基(ROS)清除系统,根部SOD、APX和CAT活性受到抑制,POD和GR受诱导活性增强,在减轻Ni胁迫引起氧化损伤方面起重要作用;Cu胁迫下骆驼蓬叶片SOD、POD、CAT、APX及GR活性较对照均有所提高,减弱了Cu胁迫引起的氧化损伤;根部APX活性受抑制,SOD、POD、CAT及GR活性受诱导增强,在缓解Cu胁迫引起氧化损伤方面发挥重要作用。Peganum harmala seeds were sown in plastic pots in a greenhouse and seedlings were treated with Ni or Cu solutions at different concentrations（50,100,200,and 400 mg/kg） and without Ni or Cu solution（control）.Ni or Cu accumulation,lipid peroxidation and the activities of antioxidative enzymes in the leaves and roots of P.harmala were examined and measured after Ni or Cu treatment.The Ni or Cu content in the leaves and roots of P.harmala increased in a dose-dependent manner.For Ni,the leaf was the preferential organ for metal storage,while for Cu,it was the root.Malondialdehyde content increased in the leaves and roots with the increase in Ni or Cu concentration,indicating lipid peroxidation increasingly aggravates with the stress.In the leaves of P.harmala,the activities of superoxide dismutase（SOD）,catalase（CAT）,peroxidase（POD）,ascorbate peroxidase（APX） and glutathione reductase（GR） increased with the increase in Ni concentration,suggesting that together they play an important role in the ROS scavenging process,while in the roots,the activities of SOD,CAT and APX decreased,but POD and GR increased,suggesting both might play an important role in weakening Ni-induced oxidative damage.The five antioxidative enzymes increased as a whole in the leaves of Cu treated P.harmala,indicating they might be exerting a certain antioxidant function,protecting seedlings from Cu-induced oxidative damage.In the roots,APX activity was reduced,but the activities of SOD,CAT,POD and GR were enhanced,suggesting they could be effective in lessening Cu-induced oxidative damage.

关 键 词：镍 铜 骆驼蓬 抗氧化酶 膜脂过氧化 

分 类 号：X173[环境科学与工程—环境科学]