难治性癫痫患者血清和脑脊液多药耐药基因-1表达的研究  

Clinical significance study of MDR1 expression in the serum and CSF of intractable epilepsy patients

作　　者：韩锟[1] 贾宁[1] 刘学文[1] 闵连秋[1] 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121001

出　　处：《中国现代医学杂志》2012年第14期89-91,共3页China Journal of Modern Medicine

摘　　要：目的探讨难治性癫痫的发病机制,为寻找难治性癫痫新的治疗方法提供理论依据。方法采用ELISA方法及荧光定量聚合酶链反应技术检测临床确诊为难治性癫痫的15例患者血清、脑脊液P-糖蛋白(P-glycoprotein,P-gp)及多药耐药基因-1(multidrug resistance1,MDR 1)-mR NA,并与用药物控制良好的15例癫痫患者和15例正常人比较。结果难治性癫痫组血清、脑脊液P-gp和MDR 1-mR NA明显高于癫痫治疗有效组和正常对照组。结论 MDR1过度表达可能是癫痫耐药的重要因素,并可作为癫痫耐药的一个客观指标。【Objective】 To investigate the pathogenesis of intractable epilepsy（IE）,it provides the theory basis for new therapy methods of IE.【Methods】 ELISA and PCR were used to test P-glycoprotein（P-gp） and multidrug resistance1-mRNA（MDR1-mRNA） in the serum and CSF of IE patients（which had been diagnosed）,and compared with epilepsy patients which reacted well to medicine and healthy subjects.【Results】 The level of P-gp and MDR1-mRNA in the serum and CSF in IE group were significantly higher than those in epilepsy group and normal control group.【Conclusions】 MDR1 expression may be an important reason for contributing to epilepsy resistance to medicine,and it can be considered as an objective index of resistance to medicine in epilepsy patients.

关 键 词：癫痫 难治性癫痫 P-糖蛋白 耐药 耐药基因-1 

分 类 号：R742.1[医药卫生—神经病学与精神病学]