光学表面等离子共振测定盐酸克伦特罗的试验研究  

Experimental study on detection of clenbuterol hydrochloride using surface plasmon resonance

作　　者：李会芹[1] 李遂亮[1] 李伟[2] 江敏[3] 魏文松[1] 胡枫江[1] 赵媛媛[1] 胡建东[1] 

机构地区：[1]河南农业大学机电工程学院,河南郑州450002 [2]河南农业大学理学院,河南郑州450002 [3]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业大学学报》2012年第2期198-202,共5页Journal of Henan Agricultural University

基　　金：国家863计划项目(2007AA100606);河南省杰出青年基金项目(104100510017);河南省教育厅创新人才项目(2009HASTIT019);郑州市科技领军人才项目(096SYJH25101)

摘　　要：为了实现高灵敏度定量检测盐酸克伦特罗,提出了一种新颖的、无标记、快速的内嵌TSPR1K23生物传感器的光学表面等离子共振(Surface plasmon resonance,SPR)盐酸克伦特罗测定方法,并通过试验验证了光学表面等离子共振生物测定装置的性能.光学表面等离子共振盐酸克伦特罗测定装置由集成光学SPR生物传感器、微流通池、便捷更换芯片的夹具、触电屏,USB接口板及光电转换电路板组成.在光学SPR芯片金膜表面固定盐酸克伦特罗抗原,在标准样品中(盐酸克伦特罗抗体质量浓度33.75 mg·L-1)分别添加0,10,20,40 mg·L-1的盐酸克伦特罗抗原,采用竞争免疫反应方法建立的盐酸克伦特罗测定标准曲线,相关系数0.997,相对标准偏差6.5%.In order to realize a high sensitive quantitative detection of residual clenbuterol hydrochloride（CLB）,an novel and label-free surface plasmon resonance（SPR） analytical approach based on the TSPR1K23 biosensor was systematically proposed,and validated experimentally for its high performance.This device is composed of an integrated biosensor,a micro-flow cell,a clamp,a touch monitor screen and an electrical control unit（ECU） incorporated with a photoelectric conversion board and a USB interface board.In this study,the measurement approach was validated using the clenbuterol hydrochloride surface antigen as an example.The antigen-CLB is immobilized to the surface of the biosensor chip by a self-assembly technology.The antigen-CLB samples at 0,10,20,40 mg·L-1 were respectively added into the standard solution of antibody-CLB（mass concentration 33.75 g·mL-1）,and a fitting curve is obtained,which optimized with a R-squared value 0.997,RSD 6.5%.

关 键 词：光学表面等离子(SPR) 盐酸克伦特罗 生物传感器 微流池 

分 类 号：S24[农业科学—农业电气化与自动化]