纤维连接蛋白、层粘连蛋白、胶原蛋白Ⅳ在大鼠膀胱癌组织的表达  

Expression of fibronectin, laminin and collagen IV in rat bladder cancer

作　　者：胡明明[1] 潘春武[1] 唐小莹[1] 胡燕琴[2] 张存明[1] 沈周俊[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院泌尿外科,200025 [2]上海交通大学医学院组织胚胎学教研室胡

出　　处：《中华实验外科杂志》2012年第6期1003-1005,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：上海高校选拔培养优秀青年教师科研专项基金资助项目（JDY10143）

摘　　要：目的观察纤维连接蛋白（FN）、层粘连蛋白（LN）和胶原蛋白Ⅳ（COLIV）在大鼠膀胱癌组织的表达。方法选取正常SD大鼠膀胱组织及大鼠原位膀胱癌模型建立后的非肌层浸润性膀胱癌（NMIBC）组织标本（每组20例），用免疫荧光及测定单位面积荧光强度（F／S）的方法比较FN、LN和COLIV表达的变化。结果FN和LN在NMIBC的表达与正常大鼠膀胱组织比较，F／S值分别为FN（6．91±1．39和0．82±0．22）、LN（6．57±2．39和0．90±0．46），差异有统计学意义（P〈0．05）；COLIV在NMIBC和正常大鼠膀胱组织F／S值为1．05±0．57和0．84±0．37，差异无统计学意义（P〉0．05）。结论FN和LN在NMIBC表达明显升高，COLIV的表达差异无统计学意义。Objective To study the expression change of extracellular matrix in rat bladder cancer. Methods The bladder specimens of normal 5D rats （ control group） and the non-muscle invasive bladder cancer （NMIBC） specimens in the established orthotopic rat model of bladder cancer （test group） （20 specimens per group） were chosen. By using immunofluoreseenee and fluorescence intensity per unit area （F/S）, the expression change of fibronectin （FN）, laminin （LN） and collagen IV was investigated. Results As compared with control group, the fluorescent staining of fihroneetin and laminin in non-muscle invasive bladder cancer was obviously increased, and the F/S values had statistically significant difference between test group and control group （PFN 〈0. 01 and PLN 〈0. 01 ） with FN （6. 91 ± 1.39 and 0. 82 ± 0. 22）, LN （6. 57 ± 2. 39 and 0. 90 ± 0. 46）, respectively. The fluorescent staining of collagen IV and the F/S value had no significant difference between test group and control group （ P 〉 0.05 ）. Conclusion The expression of fibronectin and laminin in non-muscle invasive bladder cancer was obviously increased, and there was no significant difference in the expression of COL IV between non-muscle invasive bladder 2ancer and rat bladder normal tissue.

关 键 词：膀胱癌 纤维连接蛋白 层粘连蛋白 胶原蛋白Ⅳ 

分 类 号：R737.14[医药卫生—肿瘤]