转录因子Snail及上皮钙黏素在肾透明细胞癌中的表达及其意义  

Expression and significance of Snail and E-cadherin in renal clear cell carcinoma

作　　者：王晓彬[1] 窦中岭[1] 史海军[1] 刘辉[1] 李世朋[1] 

机构地区：[1]河南科技大学第一附属医院泌尿外科,洛阳471003

出　　处：《中华实验外科杂志》2012年第6期1031-1033,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察锌指转录因子Snail及上皮钙黏蛋白（E．cadherin）在肾透明细胞癌（CCRCC）中的表达，并探讨与CCRCC病理分级、分期和转移等相关生物学行为因素的关系。方法收集我院手术切除的69例CCRCC组织标本，同时取58例癌旁组织和10例正常肾组织标本作为对照，采用免疫组织化学链霉菌抗生物素蛋白．过氧化物酶连结（sP）法检测CCRCC组织、癌旁组织和正常肾组织中Snail和E—cadherin的表达。结果CCRCC组织Snail阳性率（82．7％）显著高于癌旁组织（43．1％，P〈0．01）。CCRCC组织E—cadherin阳性率（31．9％）显著低于癌旁组织（91．5％）（P〈0．01）。两者的表达均与CCRCC不同分化程度、分期、浸润和转移有关（P〈0．05）。CCRCC组织中E—cadherin与Snail的表达呈负相关（r=-0．342，P〈0．05）。结论E—cadherin低表达与Snail高表达可能是CCRCC发生浸润转移的重要生物学标志。联合检测E—cadherin及Snail对预测CCRCC浸润转移有重要意义。Objective To study the expression of transcriptional factor Snail and adhesion factor E-cadherin in renal clear cell carcinoma （RCCC） and evaluate their correlation with pathological grading, clinical stage, invasion and metastases of RCCC. Methods The expression of Snail and E-cadherin proteins was detected by using immunohistochemical streptavidin-peroxidase method in 69 cases of RCCC tissue, 58 cases of the para-caneerous mucosa and 10 cases of normal renal tissue. Results The positive rate of Snail in RCCC was 82. 7%, which was significantly higher than that in para-cancerous mucosa （43.1% ,P 〈0. 01 ）. The positive rate of E-eadherin in RCCC was 31.9% , which was significantly lower than that in para-cancerous mucosa （91.5% ,P 〈 0.01 ）. The expression of E-cadherin and Snail was significantly related with the differential degrees, clinical stage, depth of tumor invasion and distant metastasis （P 〈 0. 05 ）. There was a negative correlation between the expression of E-eadherin and Snail in RCCC. Conclusion The over-expression of Snail and decreased expression of E-cadherin might be important biological markers for invasion and metastasis of RCCC. The combined detection of E-cadherin and Snail has far-reaching significance on predicting the invasion and metastasis of RCCC.

关 键 词：肾细胞癌 SNAIL 钙黏蛋白 

分 类 号：R737.11[医药卫生—肿瘤]