EN2蛋白在前列腺癌组织的表达及其意义  

The expression and significance of EN2 protein in human prostate cancer

作　　者：赖彩永[1] 蒋光瑜[2] 李立[3] 梁蔚波[3] 陈洁[3] 黄伟佳[4] 苏泽轩[3] 

机构地区：[1]南方医科大学研究生院,广州510515 [2]暨南大学附属第一医院临床病理科 [3]暨南大学附属第一医院泌尿外科 [4]广州医学院第三附属医院泌尿外科

出　　处：《中华实验外科杂志》2012年第6期1053-1055,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：广州市医药卫生科技项目（20121A011159）;国家高新技术研究发展计划（863计划）子课题（2007AA022006）

摘　　要：目的探讨同源异型盒蛋白EN2在前列腺癌的表达差异及其意义。方法选取48例前列腺癌、22例高级别前列腺内瘤病（HGPIN）及48例前列腺增生症（BPH），采用免疫组织化学法检测上述组织中的EN2蛋白表达差异。选取其中14例前列腺癌及15例BPH，采用Westernblot法检测EN2蛋白表达差异。结果免疫组织化学法显示EN2蛋白在前列腺癌中表达增高，主要见于前列腺腺上皮细胞质内及部分细胞核。前列腺癌、HGPIN和BPH组织中EN2蛋白表达阳性率分别为87．50％、31．81％和27．08％，采用Kruskal—WallisH检验，差异有统计学意义（P〈0．05）。EN2在高、中、低分化前列腺癌患者中阳性率分别为75．00％、88．24％和88．90％。Westernblot法检测显示EN2蛋白在前列腺癌组织的表达比BPH组织增高3倍。结论同源异型盒蛋白EN2在前列腺癌表达增高，其中以中、低分化腺癌中明显，并能为前列腺上皮细胞分泌。Objective To investigate the expression and significance of homeobox protein EN2 in human prostate cancer （PCa）. Methods EN2 expression in paraffin embedded tissue of human prostate cancer （study group, n = 48 ）, High grade prostatic intraepithelial neoplasia （HGPIN, the first control group, n = 22） and benign prostatic hyperplasia （ BPH, the second control group, n = 48） were investiga- ted by immunohistochemical. EN2 expression in fresh tissue of human prostate cancer （ study group, n = 14） and BPH （ control group, n = 15 ） were determined by Western blotting. Results The expression level of EN2 in prostate cancer tissue was higher than those in the HGPIN and BPH tissue by immunohisto- chemical. EN2 protein was presented in the cytoplasm mainly and in some cases in the nucleus. The positive rate in PCa, HGPIN and BPH were 87.50% , 31.81% and 27.08% respectively. The difference was significant statistically （P 〈 0. 01 ）. The positive rate in well-differentiated PCa （n --4）, moderately-differ- entiated PCa （n = 17） and poorly-differentiated PCa （n = 27） tissue was 75.00%, 88.24% and 88. 90% respectively. The results of Western blotting showed the expression level of EN2 in prostate cancer was 3- fold higher compare to BPH. Conclusion Homeobox protein EN2 was expressed ectopically in prostate cancer and secreted by prostate pithelial cell.

关 键 词：同源异型盒 EN2 前列腺癌 免疫组织化学 

分 类 号：R737.25[医药卫生—肿瘤]