RP-HPLC双波长切换法同时测定天舒胶囊中天麻素、阿魏酸和6,7-二羟基藁本内酯的含量  

RP-HPLC simultaneous determination of the contents of gastrodin,ferulic acid and 6,7-dihydroxyligustilide in Tianshu capsules under a changed UV wavelengths

作　　者：张晖芬[1] 陈晓辉[1] 霍艳双[1] 毕开顺[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2012年第6期443-447,共5页Journal of Shenyang Pharmaceutical University

基　　金：国家重大新药创制科技重大专项资助项目(2009ZX09301-012)

摘　　要：目的建立反相高效液相色谱-双波长切换法同时测定天舒胶囊中天麻素、阿魏酸和6,7-二羟基藁本内酯的含量。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-体积分数0.1%乙酸水溶液为流动相,梯度洗脱,流速为1.0 mL.min-1,检测波长为270 nm(天麻素)、320 nm(阿魏酸和6,7-二羟基藁本内酯),柱温35℃。结果在15 min内天舒胶囊中天麻素、阿魏酸和6,7-二羟基藁本内酯质量浓度分别在30.50～305.0 mg.L-1(r=0.999 2)、34.96～349.6 mg.L-1(r=0.999 7)和46.54～465.4 mg.L-1(r=0.999 4)内与峰面积线性关系良好;平均加样回收率分别为98.8%、98.7%和98.2%,RSD分别为1.9%、1.2%和2.1%。结论本方法操作简便,结果准确,与《中华人民共和国药典》所述方法相比,增加了含量测定指标,提高了全面控制天舒胶囊质量的水平。Objective To establish a reversed-phase HPLC method for simultaneous determination of gastrod- in, ferulic acid and 6,7-dihydroxyligustilide in Tianshu （Chinese medicines）capsules. Methods A Kromasil C18 column（250 mm ×4. 6 mm,5 μm）was used with a methanol-0. 1% acetic acid water solution gradient elution system. The elution were detected at 270 nm for gastrodin from 0- 8.0 min and at 320 nm for ferulic acid and 6,7-dihydroxyligustilide from 8. 1- 15 min. The flow rate was 1.0 mL. min - 1 and the column tem- perature was 35 ~C. Results The linear range Of gastrodin, ferulic acid and 6,7-dihydroxyligustilide were 30. 50-305.0 mg. L - 1 （ r = 0. 999 2）, 34. 96- 349. 6 mg. L ^- 1 （ r = 0. 999 7 ） and 46. 54- 465.4 mg- L^ -1 （ r = 0. 999 4） ,respectively. The average recoveries （ n = 9 ） of the three components were 98.8%, 98.7%, 98.2%, and RSD were 1.9%, 1.2%, 2. 1%, respectively. Conclusions The method is accurate, sensitive and reliable for determination of gastrodin, ferulic acid and 6,7-dihydroxyligustilide. Compared with that in the Chinese Pharmacopoeia（2010） ,the method add a new marker ingredient into the assay content and im- prove the quality standard of Tianshu capsules.

关 键 词：天舒胶囊 天麻素 阿魏酸 6 7-二羟基藁本内酯 RP-HPLC 含量测定 

分 类 号：R917[医药卫生—药物分析学]