云南地区三七和草果上真菌污染的初步分析  

Primary investigation of contaminating fungi on Panax notoginseng and Amomum tsaoko in Yunnan

作　　者：宋美芳[1] 陈娟[2] 李学兰[1] 唐德英[1] 孙炳达[3] 高微微[1,2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所云南分所,云南景洪666100 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193 [3]中国科学院微生物研究所菌种保藏中心,北京100101

出　　处：《中国中药杂志》2012年第12期1734-1737,共4页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09502-025-01);国家中医药管理局中医药行业科研专项(200807042)

摘　　要：目的:了解云南地区主产的中药材三七和草果上污染真菌的主要类群,分析这2种常用药材上潜在的真菌毒素污染的可能性。方法:采集来源于云南不同地区市售的三七、草果药材,使用稀释平板法分离药材表面真菌,依据形态学特征结合分子生物学方法对主要分离菌株进行鉴定。结果:三七污染真菌以淡紫拟青霉和橘青霉为优势菌;草果污染菌中的优势菌为淡紫拟青霉和黄曲霉。结论:根据三七和草果上污染菌的情况,在云南地区三七存在橘青霉毒素污染的风险,草果存在黄曲霉毒素污染的风险。Objective ： To analyze the potential risks of fungal contaminants on Panax notoginseng and Amomum tsaoko. Meth- od： The primary investigation was conducted in the P. notoginseng and A. tsaoko major production areas in Yunnan. Samples of P. notoginseng and A. tsaoko were collected from drugstores and markets in 3 cities of Yunnan. Dilution-plate method was applied for the isolation of fungi, the obtained species were identified according to morphological and molecular approaches. Result： Paecilomyces lilacinus and PeniciUium citrinum were dominant on samples of Panax notoginseng. P. lilacinus and AspergiUusflavus were dominant on samples of Amomum tsaoko. Conclusion： In Yunnan province, the major fungal contaminants on P. notoginseng are P. lilacinus and P. citrinum and the major fungal contaminants on A. tsaoko are P. lilacinus and AspergiUusflavus. There exists a potential contamina- tion risk of citrinin on P. notoginseng and aflatoxin on A. tsaoko.

关 键 词：三七 草果 污染真菌 真菌毒素 云南 

分 类 号：R282[医药卫生—中药学]