壬基酚降解菌产酶条件的优化  

Optimization of conditions on nonylphenol-degrading enzyme production

作　　者：张美珍[1] 邓国志[1,2] 张小凡[1] 何义亮[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240 [2]安徽大学资源与环境工程学院,安徽合肥230601

出　　处：《环境污染与防治》2012年第6期46-50,共5页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.50978161);"十一五"国家重大科技水专项(No.2008ZX07211-002)

摘　　要：通过单因素试验和正交试验确定了实验室保藏的壬基酚降解菌株沙雷氏菌(Serratiasp.LJ)的最佳产酶条件:以壬基酚、硫酸铵为碳源、氮源,培养基初始pH为6.8,培养温度为30℃,种子活化时间为24h,接种量为3%(体积分数)。在此条件下培养72h后,最高酶活力为1.314IU/mL,是优化前的1.77倍。The optimal conditions of nonylphenol-degrading strains named Serratia sp. LJ for enzyme production were obtained by means of single factor analysis and orthogonal design. Results showed that the optimal enzyme pro duction conditions were applying nonylphenol, ammonium sulfate as carbon and nitrogen sources, cultured under ini tial pH of 6.8 at .30 ℃, gave the seed activation time of 24 h and inoculum of 3%. Under these optimal conditions for 72 h,the enzyme activity was up to 1. 314 IU/mL, 1.77 times as high as the original activity.

关 键 词：壬基酚降解菌 酶活 产酶条件 

分 类 号：X172[环境科学与工程—环境科学]