Rapid Determination of Costunolide and Dehydrocostuslactone in Human Plasma Sample and Chinese Patent Medicine Xiang Sha Yang Wei Capsule Using HPLC-DAD Coupled with Second-order Calibration  

作　　者：刘亚娟 吴海龙 朱绍华 康超 许慧 苏志义 谷惠文 俞汝勤 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha,Hunan 410082,China [2]Hunan Entry-Exit Inspection and Quarantine Bureau,Changsha,Hunan 410082,China

出　　处：《Chinese Journal of Chemistry》2012年第5期1137-1143,共7页中国化学（英文版）

摘　　要：A novel methodology that combines high performance liquid chromatography with photodiode-array detector （HPLC-DAD） coupled with second-order calibration method based on alternating trilinear decomposition （ATLD） algorithm was used in determination of the effective constituents such as costunolide and dehydrocostuslactone, in plasma sample and Chinese patent medicine Xiang Sha Yang Wei （XSYW） capsule. Complicated systems such as plasma and Chinese patent medicine which have intricate components are tedious to isolate and purify. The problem that chromatographic peaks are heavily overlapped among the analytes and interferents from the background matrices can be resolved, and the satisfactory quantification results have been gained with the help of the ATLD algorithm which utilized ＂mathematical separation＂ instead of partial ＂physical or chemical separation＂. Meanwhile, HPLC-MS/MS method was used to validate the accuracy of the proposed determination method.A novel methodology that combines high performance liquid chromatography with photodiode-array detector （HPLC-DAD） coupled with second-order calibration method based on alternating trilinear decomposition （ATLD） algorithm was used in determination of the effective constituents such as costunolide and dehydrocostuslactone, in plasma sample and Chinese patent medicine Xiang Sha Yang Wei （XSYW） capsule. Complicated systems such as plasma and Chinese patent medicine which have intricate components are tedious to isolate and purify. The problem that chromatographic peaks are heavily overlapped among the analytes and interferents from the background matrices can be resolved, and the satisfactory quantification results have been gained with the help of the ATLD algorithm which utilized ＂mathematical separation＂ instead of partial ＂physical or chemical separation＂. Meanwhile, HPLC-MS/MS method was used to validate the accuracy of the proposed determination method.

关 键 词：COSTUNOLIDE dehydrocostuslactone HPLC-DAD second-order calibration alternating trilinear decomposition 

分 类 号：O657.72[理学—分析化学] U469.6[理学—化学]