持续性低眼压动物模型的制备  

Establishment of persistent ocular hypotony animal model with CO_2 laser sclerectomy

作　　者：戴淑真[1] 王丽娅[1] 张效房[2] 张金嵩[2] 

机构地区：[1]河南省眼科研究所,郑州450003 [2]郑州大学第一附属医院

出　　处：《中国实用医药》2012年第13期34-35,共2页China Practical Medicine

基　　金：河南省卫生厅科技攻关项目(项目编号:200803026;200903057)

摘　　要：目的探讨建立低眼压动物模型的方法。方法 16只青紫兰兔均以右眼为实验眼,采用CO2激光外路巩膜切除术的方法建立持续性低眼压模型,左眼为正常对照眼,不做任何处理。巩膜切除术前及术后1、7、14、21、28d测量所有动物双眼眼压,观察兔眼功能性滤过泡形成情况、眼前节的炎症反应及眼底变化,应用SPSS17.0统计软件包对实验眼和对照眼的眼压行配对t检验。结果 CO2激光外路巩膜切除术后兔眼压大幅度下降,随着低眼压时间的延长,兔眼压有升高的趋势,至低眼压21d,所有兔眼压仍均低于8mmHg,术后各时间点实验眼与对照眼眼压相比差异有统计学意义(P<0.01)。CO2激光外路巩膜切除术后兔眼功能性滤过泡形成良好,前节反应轻微,7d后所有炎症反应均消失;术后第7天眼底检查可见视乳头水肿,至低眼压21d水肿最明显。结论 CO2激光外路巩膜切除术能有效建立低眼压动物模型,操作简便,降压效果确切,术后炎症反应轻微。Objective This work was to explore the effecacy and safety of the method to establish the persistent ocular hypotony animal model. Methods Sixteen healthy black and blue rabbits were chosen. The persistent ocular hypotony model was established in the right eyes of all the rabbits with CO2 laser sclereetomy and the left eyes was left untreated as normal control eyes. The intraocular pressure（IOP） of all the eyes was measured before operation and on the first day, seventh day, fourteenth day, twenty-first day, twenty-eighth day after operation respectively. The formation of functional filtering bleb, the reaction of anterior chamber and fun- dus was also observed. All the data were expressed by x ~ s and analyzed statistically by t test. Results The IOP of the experimental eyes was decreased dramatically after C02 laser sclerectomy. The IOP was still below 8 mm Hg 21 days afer operation. Statistically difference was found between the experimental eyes and the normal contral eyes in all the time point after the operation （P 〈 0. 01 ）. The functional filtering bleb was well after op- eration. No seriou anterior chamber reaction was observed in all the experimental eyes. The papilledema was observed 7 days after operation and became most obvious 21 days after operation. Conclusion CO2 laser sclerec- tomy is the effective, simple and safe method to establish the persistent ocular hypotony model.

关 键 词：持续性低压眼 动物模型 眼前节 眼底 

分 类 号：R-332[医药卫生]