血管内皮生长因子和碱性成纤维细胞生长因子在乳房外Paget病中的表达和意义  

The expression and clinical significance of vascular endothelial growth factor and basic fibroblast growth factor in extramammary Paget disease

作　　者：许筱云[1,2] 陈晶[3] 杨杰[4] 宋宁静 任勇亚[1] 冯振卿[1] 

机构地区：[1]南京医科大学病理学系,江苏南京210029 [2]江苏省省级机关医院皮肤科,江苏南京210024 [3]南京医科大学附属南京第一医院甲乳外科,江苏南京210006 [4]南京医科大学第一附属医院泌尿外科,江苏南京210029 [5]上海市皮肤病研究所.上海200050

出　　处：《南京医科大学学报（自然科学版）》2012年第5期664-668,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家"973"项目(2010CB933902)

摘　　要：目的:研究在乳房外Paget病(extramammary Paget disease,EMPD)组织及相应病灶旁正常皮肤黏膜组织中血管内皮生长因子(vascular endothelial growth factor,VEGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)蛋白表达之间的差异,并探讨它们在EMPD组织血管形成中的相互关系。方法:采用免疫组化法研究9例EMPD组织及相应病灶旁正常皮肤黏膜组织中VEGF及bFGF蛋白的表达,并结合临床资料进行统计分析。结果:通过配对t检验分析免疫组化染色检测结果,发现EMPD组织中VEGF及bFGF蛋白染色信号(信号评分)均显著强于癌旁正常皮肤黏膜组织(P<0.05)。以bFGF和VEGF基因蛋白免疫组化染色"信号评分比值"(bFGF/VEGF)进行配对t检验发现,EMPD组织两基因蛋白"信号评分"比值显著高于癌旁正常黏膜组织(t=2.630,P=0.030)。结论:VEGF和bFGF基因的表达产物是EMPD肿瘤血管形成中的关键信号蛋白,其相互作用提供的刺激信号在EMPD的发生、发展中起着促进作用,尤其是bFGF基因的高表达可能起到更为关键的作用。Objective：To study the expression differences of vascular endothelial growth factor（VEGF） and basic fibroblast growth factor（bFGF） protein in extramammary Paget disease（EMPD） tissues and corresponding adjacent normal skin and mucosa tissues,and explore their relationship in angiogenesis of EMPD tissue.Methods：We detected VEGF and bFGF protein expression by immunohistochemical staining in EMPD tissue and corresponding adjacent normal skin and mucosa from nine cases and combined their clinical data to perform statistical analysis.Results：We found that VEGF and bFGF protein staining signals（score） in EMPD tissues were significantly stronger than that of the adjacent normal skin and mucosa（P 0.05）.Furthermore,we found that ＂signal rate ratio＂ of the two proteins（bFGF/VEGF） was significantly higher in EMPD tissues than that of adjacent normal mucosa（t = 2.630,P = 0.030）.Conclusion：VEGF and bFGF gene products are key signaling proteins in EMPD angiogenesis,and the interaction of the two stimulus signals plays a facilitating role in the occurrence and development of EMPD,especially the high expression of bFGF.How to block the over-expression of VEGF and bFGF in EMPD to destroy the blood supply of tumor cells and restrain the rapid occurrence and development of tumor is still the next focus of our study.

关 键 词：乳房外PAGET病 VEGF BFGF 血管形成 

分 类 号：Q786[生物学—分子生物学] R739.5[医药卫生—肿瘤]