六种小鼠肿瘤及白血病细胞株的细胞动力学研究  

Cell Kinetics of Six Strains of Murine Tumor and Leukemia Cell Lines

作　　者：万景华[1] 张淑靖[1] 糜静娴[1] 

机构地区：[1]中国医学科学院血液学研究所

出　　处：《中国肿瘤临床》1990年第2期120-122,109,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金

摘　　要：本文对L7811-85,L7212-85,L1210-86,P388-86 4个淋巴细胞白血病及S180-86和肝Ca-86肿瘤细胞株分别测定了细胞生长曲线、克隆形成效率和细胞DNA含量分布等参数。对其中L7811-85细胞株进行了较系统的细胞动力学研究,并应用~3H-TdR自杀试验微培养测定其克隆形成细胞的增殖时和合成时。结果表明:这6种细胞株均可在体外无刺激因子作用下良好生长。并可见随细胞传代数增加,细胞增殖加速,克隆形成率提高。用~3H-TdR自杀试验测出的克隆形成细胞增殖时与群体细胞倍增时相比,可反映其潜在倍增时。Determination of cell growth curves, cloning efficiencies and analysis of DNA content distribution by flow cytometry were carried out on murine leukemia L7811-85, L7212-85, L1210-86, P388-86 cell lines and S180-86, Mepatoma-86 cell lines. In addition, the generation time and synthesis time of clone forming cells of L7811-85 were determined by ^(3)H-TdR suicide analysis in microculture. The cell kinetics parameters showed that the 6 cell lines could grow well without any stimulating factors in vitro. As the generations of cell lines increased in number, cell growth was accelerated, generation time shortened and cloning efficiency enhanced. The generation time of clone forming cells of L7811-85 determined by ^(3)H-TdR suicide analysis may reflect the potential doubling time of total cell population.

关 键 词：细胞动力学 小鼠 白血病细胞株 

分 类 号：R73－354[医药卫生—肿瘤]