外源总DNA直接导入植物的分子育种研究进展  

Research Advances in the Molecular Breeding by Introducing Exogenous Total DNA into Plant Directly

作　　者：肖靖[1] 高飞[1] 谷运红[1] 

机构地区：[1]郑州大学河南省离子束生物工程重点实验室,河南郑州450052

出　　处：《安徽农业科学》2012年第17期9211-9213,9240,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(10505018);农业部农业公益性行业科研专项经费项目(200803034);河南郑州市科技局攻关项目(083SGYS33261-10)

摘　　要：文中介绍了2种较常用的外源总DNA直接导入植物的方法—花粉管通道法和离子束介导法,分别从原理与方法、发展与应用、机理与验证、问题与展望4个方面综述了这2种方法的创立与发展以及应用此方法取得的研究成果。Two commonly used techniques of introducing exogenous total DNA into plant directly were introduced,which were pollen - tube path- way method and ion beam implantation method. The establishment, development and application achievements of the two techniques were intro- duced from their principles and methods, development and application, mechanism and verification, problems and prospect.

关 键 词：外源总DNA 植物 花粉管通道 离子束介导 分子育种 

分 类 号：S188[农业科学—农业基础科学]