盐胁迫对3种甘薯品种抗氧化酶及可溶性蛋白含量的影响  被引量:8

Effects of NaCl Stress on Antioxidant Enzymes Activities and Soluble Protein Content in 3 Kinds of Sweet Potatoes(Dioscorea esculenta)

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作  者:蔡翔君[1] 关诗扬[1] 过晓明[1] 

机构地区:[1]江苏师范大学生命科学学院,江苏徐州221116

出  处:《安徽农业科学》2012年第17期9228-9229,共2页Journal of Anhui Agricultural Sciences

摘  要:[目的]研究NaCl胁迫对3个品种甘薯(Dioscorea esculenta)抗氧化酶和可溶性蛋白含量的影响,探讨其耐盐性机理。[方法]以徐薯25、徐薯26和徐薯18为材料,分析浇灌不同浓度(0、50、100、150、200 mmol/L)NaCl后其幼苗超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、丙二醛(MDA)含量及可溶性蛋白含量。[结果]随着NaCl浓度增大,徐薯25、徐薯26和徐薯18幼苗的SOD活性、CAT活性和可溶性蛋白质含量呈现先增后减的趋势,而MDA含量均呈现上升的趋势。其中徐薯18的各项指标与其他2个品种存在很大差异,显示出良好的耐盐能力。[结论]徐薯18的耐盐性最高,徐薯26次之,徐薯25较差,这可为甘薯耐盐栽培和扩大种植提供科学指导。[Objective] The purpose was to study the effects of NaC1 stress on antioxidant enzymes activities and soluble protein content in 3 kinds of sweet potatoes, and discuss their salt resistance mechanism. [ Method ] Activities of superoxide dismutase (SOD) and catalase (CAT), and contents of malondial dehyde (MDA) and soluble protein of 3 sweet potatoes,Xushu 25,Xushu 26 and Xushu 18, treated with different con- centrations (0,50,1(30,150,200 mmol/L) NaC1 solution,were analyzed. [ Result] The results showed that with the increase of NaC1 concentra- tions, the activities of SOD and CAT and soluble protein content of 3 sweet potatoes showed a change a trend of increasing firstly and then decrea- sing,while their MDA contents all showed change trend of increasing all the time. Thereinto,Xushu 18 showed a significant difference compared with the other two sweet potatoes,and had a good salt resistance. [ Conclusion] Three sweet potatoes'salt resistance is as follows: Xushu 18 〉 Xushu 26 〉 Xushu 25 .which provides a scientific guidance for their salt resistance cultivation and enlar^in~ planting.

关 键 词:甘薯 盐胁迫 抗氧化酶 可溶性蛋白质 

分 类 号:S632[农业科学—蔬菜学]

 

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