不同产地南丰蜜桔果实品质和蔗糖代谢相关酶研究  

Study on Fruit Qualities and Sucrose-metabolizing Enzymes in Citrus reticulata Blanco var.kinokuni(Tanaka)H.H.Hu from Six Different Places

作　　者：丁明华[1] 涂艺声[1] 

机构地区：[1]江西师范大学生命科学学院,江西南昌330022

出　　处：《安徽农业科学》2012年第17期9233-9235,9238,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30660020);江西省科技支撑计划项目[赣财教2007(173)]资助

摘　　要：[目的]探索不同产地南丰蜜桔果实品质的差异及其与蔗糖代谢相关酶活性的关系,为指导南丰蜜橘的适度生产、保持地域产品声誉以及科学发展名贵稀有物种提供参考依据。[方法]研究分析了6个不同产地南丰蜜桔果实的主要品质特性和蔗糖代谢相关酶活性特征。[结果]南丰原产地的南丰蜜桔中总糖含量、尤其还原糖含量、糖酸比、固酸比均优于其他产地的果品,故体现出品质优异;不同产地南丰蜜桔中积累的可溶性糖种类含量存在差异;通过对蔗糖中性转化酶(NI)活性与还原糖含量、蔗糖合成酶(SS)活性和蔗糖磷酸合成酶(SPS)活性与蔗糖含量的相关性分析发现,还原糖与NI活性存在极显著正相关,蔗糖含量与SPS和SS活性之和存在显著相关性。[结论]该研究结果为南丰蜜桔引种种植范围的界定及品质的保证提供了参考依据。[ Objective ] To explore the different fruit quality of C. reticulate,and its relations with sucrose-metabalizing enzymes,so as to provide references for the appropriate production,protecting the brand and developing the rare species of C. reticulate. [ Method] The quality characteris- tics and sucrose-metabolizing enzymes in C. reticulate from six different places were studied. [ Result ] The C. reticulate produced in Nanfeng County had higher contents of total sugar, especially reducing sugar, sugar-acid ratio, and solid-acid ratio than that produced in other regions, it re- flected superior quality. The types and contents of soluble sugar were different in different areas. Through the correlation analysis of the sucrose neutral invertase （NI） activity and the reducing sugar,sucrose synthase （SS） ,sucrose phosphate synthase （SPS） activity and sucrose content,the content of reducing sugar was.positively correlated with N1 activity, sugar content was significantly correlated with SPS and SS activity. [ Conclu- sion ] The study results provided references for the planting area and quality of C. reticulate.

关 键 词：南丰蜜桔 果实品质 中性转化酶 蔗糖合成酶 蔗糖磷酸合成酶 

分 类 号：S666.2[农业科学—果树学]