类黄酮异戊烯转移酶基因在酿酒酵母中的表达  

Expression Analysis of Flavonoid Prenyltransferase-like Genes in Saccharomyces Cerevisia

作　　者：袁田田[1] 高娟[2] 刘小烛[1] 

机构地区：[1]西南林业大学林学院,云南昆明650224 [2]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650204

出　　处：《安徽农业科学》2012年第18期9596-9597,9600,共3页Journal of Anhui Agricultural Sciences

基　　金：云南省高等学校本科实验教学示范中心"林木生物技术实验中心";"生物学"云南省高校优势重点学科及西南林业大学"大型仪器设备共享平台"资助

摘　　要：[目的]对类黄酮异戊烯转移酶基因在酿酒酵母中的表达进行研究。[方法]以粗毛淫羊藿为材料,对其类黄酮异戊烯转移酶基因(EaPT1)进行克隆,并构建重组质粒,将其转化至酿酒酵母INVSc1中表达,进行黄酮类底物粗酶检测。[结果]获得了大小约1 200 bp的EaPT1基因;经SDS-PAGE检测,重组质粒转化后的酵母菌有疑似EaPT1的表达产物;粗酶检测中未能检测到EaPT1酶活性。[结论]为粗毛淫羊藿植物次生代谢,特别是类黄酮生物合成途径的解析奠定基础。[ Objeetive ] The aim was to study the expression of flavonoid prenyhransferase-like genes in Saccharomyces cerevisia. [ Method ] U- sing the Epimedium acuminatum as material, the flavonoid prenyhransferase-like gene （EaPT1） was cloned, and with the construction of re- combinant plasmid which expressed in Saccharomyces cerevisia, the crude enzyme was detected by flavonoid substrate. [ Result ] An about 1 200 bp EaPTI gene was obtained; through the SDS-PAGE detection, the Saccharomyces cerevisia which had be transformed in recombinant plasmid had as if EaPTI＇s expression product; there was no EaPT1 enzymatic activity in the crude enzyme detection. [ Conelusion] It pre- pared the ground for the analysis of secondary metabolism of Epimedium acuminatum, specially the synthetic route of flavonoid organism.

关 键 词：淫羊藿苷 重组质粒 异戊烯转移酶 酿酒酵母 

分 类 号：S188[农业科学—农业基础科学]