应用多个胃癌组织提取物通过鞣化羊红细胞电泳以提高对胃癌的诊断  

The Value of Fixed Tanned Erythrocyte Electrophoretic Mobility with Extracts from Multiple Gastric Cancer in the Diagnosis of Gastric Cancer

作　　者：房殿春[1] 刘为纹[1] 晋华源 袁爱力[1] 

机构地区：[1]第三军医大学附属第一医院消化科

出　　处：《中国肿瘤临床》1990年第3期153-156,共4页Chinese Journal of Clinical Oncology

摘　　要：以正常胃粘膜和5个胃癌的3MKCL提取物作抗原应用FTEEM试验对149例胃癌、良性胃病、其它肿瘤及正常人进行测检。以正常胃粘膜作抗原,各受试组FTEEM仅出现个别阳性。应用单个胃癌抗原检测胃癌病人阳性率为71.43％,而用5个胃癌抗原阳性率可提高到90.49％。应用胃癌提取物作抗原在其它肿瘤病人中均存在不同程度的FTEEM交叉反应。结果表明,应用多种胃癌提取物作抗原可提高胃癌患者FTEEM阳性率。如能进一步扩大抗原成分,并对抗原进行各种非特异性物质的吸收纯化,将会提高其特异性和敏感性。To improve the specificity and sensitivity of fixed tanned erythrocyte electrophoretic mobility (FTEEM) assay for the detection of gastric cancer, extracts from 5 different gastric cancer tissues were used for the assay in 149 cases including 21 cases of gastric cancer, 33 cases of other cancers, 75 cases of benign diseases and 20 healthy volunteers. As compared with normal control, retardation of mobility rate≥15％ was defined as positive. Under circumstance when single tumor extract was used, FTEEM was positive in 71.43％ in patients with gastric cancer, being significantly higher than those of other groups of patients (6.4-27.69％, P<0.01). The positive rate was elevated to 90.48％ in patients with gastric cancer if five tumor extracts were employed. Furthermore, considerable cross reactivity was found in some patients with other cancers, and increased as more tumor extracts were used. The results indicated that the tissue extracts of gastric cancer might contain tumor-associated antigens in soluble form, and the specificity and sensitivity of FTEEM assay could be improved by using multiple tumor extracts.

关 键 词：胃癌 鞣化羊红细胞 电泳 抗原 

分 类 号：R735.204[医药卫生—肿瘤]