Antibacterial activity of calcium hydroxide combined with chitosan solutions and the outcomes on the bond strength of RealSeal sealer to radicular dentin  

作　　者：Shaymaa Elsayed Elsaka Amr Mohamed Elnaghy 

机构地区：[1]Department of Dental Biomaterials,Faculty of Dentistry,Mansoura University,Mansoura,PC 35516,Egypt [2]Department of Conservative Dentistry and Endodontics,Faculty of Dentistry,Mansoura University,Mansoura,PC 35516,Egypt

出　　处：《The Journal of Biomedical Research》2012年第3期193-199,共7页生物医学研究杂志（英文版）

摘　　要：The purpose of this study was to investigate the antibacterial activity of calcium hydroxide [Ca（OH）2] combined with chitosan solutions against Enterococcus faecalis-infected root canal dentin and the effect of this new intraca- nal medicament on the bond strength of RealSeal sealer to radicular dentin. An experimental intracanal medicament was prepared by mixing different concentrations of chitosan solution （25%, 50%, and 100%, W/V） to Ca（OH）2 powder. Antibacterial activity was evaluated and the total numbers of colony forming units were determined. Bonding ability of RealSeal sealer to radicular dentin was evaluated using push-out bond strength test. Data were analyzed using one-way analysis of variance （ANOVA） and Tukey＇s multiple comparison tests. We found that Ca（OH）2 combined with different concentrations of chitosan solutions showed better antibacterial activity than Ca（OH）2 mixed with saline, without significantly affecting the bond strength of RealSeal sealer to radicular dentin （P 〉 0.05）. The findings suggest that Ca（OH）2 combined with chitosan is a promising intracanal medicament and may be effective in endodontic therapy.The purpose of this study was to investigate the antibacterial activity of calcium hydroxide [Ca（OH）2] combined with chitosan solutions against Enterococcus faecalis-infected root canal dentin and the effect of this new intraca- nal medicament on the bond strength of RealSeal sealer to radicular dentin. An experimental intracanal medicament was prepared by mixing different concentrations of chitosan solution （25%, 50%, and 100%, W/V） to Ca（OH）2 powder. Antibacterial activity was evaluated and the total numbers of colony forming units were determined. Bonding ability of RealSeal sealer to radicular dentin was evaluated using push-out bond strength test. Data were analyzed using one-way analysis of variance （ANOVA） and Tukey＇s multiple comparison tests. We found that Ca（OH）2 combined with different concentrations of chitosan solutions showed better antibacterial activity than Ca（OH）2 mixed with saline, without significantly affecting the bond strength of RealSeal sealer to radicular dentin （P 〉 0.05）. The findings suggest that Ca（OH）2 combined with chitosan is a promising intracanal medicament and may be effective in endodontic therapy.

关 键 词：ANTIBACTERIAL calcium hydroxide CHITOSAN Enterococcusfaecalis push-out RealSeal 

分 类 号：R783.1[医药卫生—口腔医学]