结直肠腺癌中Galectin-3、Fascin-1和β-catenin蛋白表达与其临床病理特征的关系  

The Expressions of Galectin-3,Fascin-1,and β-catenin Protein in Colorectal Adenocarcinoma and Their Relations to Clinicopathologic Characteristics

作　　者：李宏伟[1] 张海[1] 许若飞[1] 黄勇[1] 王铁山[1] 胡海霞[1] 熊正文[1] 

机构地区：[1]解放军第251医院北京军区肿瘤病理技术研究中心,河北张家口075000

出　　处：《中国普外基础与临床杂志》2012年第4期410-415,共6页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨Galectin-3、Fascin-1和β-catenin蛋白在结直肠腺癌(colorectal carcinoma,CRC)组织中的表达及其与临床病理特征的关系。方法应用微波-EliVisionTM(MW-EliVisionTM)免疫组织化学染色技术检测60例CRC、30例结直肠腺瘤和30例正常结直肠黏膜组织中Galectin-3、Fascin-1和β-catenin蛋白的表达,分析其与CRC临床病理特征的关系。结果 Galectin-3、Fascin-1和β-catenin蛋白表达阳性率在CRC组织中分别为68.3%(41/60)、53.3%(32/60)和81.7%(49/60);在腺瘤组织中分别为46.7%(14/30)、30.0%(9/30)和43.3%(13/30);在正常黏膜组织中分别为20.0%(6/30)、3.3%(1/30)和13.3%(4/30),三者在三种组织中表达阳性率间的差异均有统计学意义(P<0.05)。三者的表达与CRC的TNM分期、浸润程度和淋巴结转移有关(P<0.05,P<0.01);Galectin-3和β-catenin蛋白的表达与CRC分化程度有关(P<0.05),而Fascin-1蛋白表达与CRC分化程度无关(P>0.05);三者的表达均与患者年龄、性别及肿瘤大小无关(P>0.05)。Galectin-3蛋白与Fascin-1和β-catenin蛋白表达呈正相关(r=0.728,P<0.01;r=0.696,P<0.01);β-catenin蛋白与Fascin-1蛋白表达呈正相关(r=0.507,P<0.01)。结论 Galectin-3、Fascin-1和β-catenin蛋白在CRC组织中的高表达与其高侵袭能力和淋巴结转移有一定相关性,可作为潜在预测结直肠癌浸润和转移的指标。Objective To explore the expressions of Galectin-3,Fascin-1,and β-catenin protein in colorectal adenocarcinoma and the relations to clinicopathologic characteristics.Methods The expressions of Galectin-3,Fascin-1,and β-catenin protein were detected in 60 cases of colorectal adenocarcinoma,30 cases of adenoma,and 30 cases of normal mucosa by microwave-EliVisionTM immunohistochemistry method,and analyzed the expressions of them and the relations to clinicopathologic characteristics.Results The expression rate of Galectin-3,Fascin-1,and β-catenin protein in CRC was 68.3%（41/60）,53.3%（32/60）,and 81.7%（49/60） respectively,which was 46.7%（14/30）,30.0%（9/30）,and 43.3%（13/30） respectively in adenoma,and 20.0%（6/30）,3.3%（1/30）,and 13.3%（4/30） respectively in normal mucosa,the differences had statistical significance（P〈0.05）.The expressions of Galectin-3,Fascin-1,and β-catenin protein had statistically significant correlation with the TNM stage,invasive degree,and lymph node metastasis of colorectal adenocarcinoma（P〈0.05,P〈0.01）.The expressions of Galectin-3 and β-catenin protein had statistically significant correlation with the different differentiation degree of colorectal adenocarcinoma（P〈0.05）,but the expression of Fascin-1 protein was not related to differentiation degree of colorectal adenocarcinoma（P〉0.05）.The expressions of Galectin-3,Fascin-1,and β-catenin protein had not statistically significant correlation with the patient’s age and gender,and tumour size（P〉0.05）.There were positive correlations between the Galectin-3 and Fascin-1 or β-catenin（r=0.728,P〈0.01;r=0.696,P〈0.01）,and there was positive correlation between β-catenin and Fascin-1（r=0.507,P〈0.01）.Conclusions The high expressions of Galectin-3,Fascin-1,and β-catenin protein in colorectal adenocarcinoma tissues are some extent correlated to the high invasive ability and lymph node metastasis,which could be used for the indexes to predict the invasion and metastas

关 键 词：GALECTIN-3蛋白 Fascin-1蛋白 Β-CATENIN蛋白 结直肠肿瘤 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]