转化生长因子受体Ⅱ与去神经性肌萎缩的相关性研究  

Correlation between TβRⅡ and Denervation-induced Skeletal Muscle Atrophy

作　　者：王旭[1,2] 王璐[3] 刘红菊[3] 李莉[3] 李景龙[3] 范明[1] 陈晓萍[3] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850 [2]沈阳军区总医院医学实验科,辽宁沈阳110840 [3]中国航天员科研训练中心,北京100094

出　　处：《航天医学与医学工程》2012年第3期217-219,共3页Space Medicine & Medical Engineering

基　　金：国家重点基础研究973项目(2011CB711000);国家自然科学基金面上项目(31171144)

摘　　要：目的研究去神经致肌萎缩和纤维类型转化的分子机制,探讨TβRII-SMAD信号通路在此过程中的相关性。方法取10只C57BL/6小鼠,右后肢坐骨神经离断,左后肢假手术对照。Real Time PCR(RT-PCR)检测术后14 d比目鱼肌和跖肌萎缩基因MAFbx,MuRF-1,及PGC-1α,MHCIIb,TβRII的表达变化。结果去坐骨神经后萎缩基因MAFbx,MuRF-1及MHCIIb在比目鱼肌和跖肌明显上调;PGC-1α,TβRII的表达在比目鱼肌和跖肌呈现差异性变化。结论 MAFbx,MuRF-1和PGC-1α在肌萎缩过程中发挥重要作用,TβRII可能参与了对肌萎缩的调节。Objective To research the mechanisms of the denervation-induced muscle atrophy, fiber-type switc- hing, and the correlation between TβRII-SMAD and its mechanisms. Methods Sciatic nerves were cut off by 5 mm in the right hind legs of 10 C57BL/6 mice with the sham on the left. QRT-PCR was performed 14 d later for quantification of mRNA expression of genes： MAFbx, MuRF-1, PGC-1α, MHCIIb, and TβRII. Results Relative mRNA levels of MAFbx, MuRF-1, MHCIIb in denervated Sol and Plan muscles were significantly increased and the change of the genes, PGC-1α and TβRII, were significant in Sol and Plan muscles when com- pared with those in sham-operated contralateral muscles. Conclusion Genes MAFbx, MuRF-1α and PGC-1 play important roles in muscular atrophy, and the gene TβRII may be involved in the adjustment course of it.

关 键 词：去神经 肌萎缩 TΒRII 分子机制 

分 类 号：R854[医药卫生—航空、航天与航海医学]