2-脱氧-D-葡萄糖对非霍奇金淋巴瘤细胞株Namalwa和SU-DHL-4糖酵解通路的干预研究  

The effect of 2-deoxy-D-glucose as a glycolysis pathway antagonist on non-Hodgkin′s lymphoma cell lines in vitro

作　　者：庞淯阳[1] 王婷[1] 陈芳源[1] 钟华[1] 钟济华[1] 

机构地区：[1]上海交通大学医学院附属仁济医院血液科白血病研究室,上海200127

出　　处：《诊断学理论与实践》2012年第2期116-120,共5页Journal of Diagnostics Concepts & Practice

基　　金：国家自然科学基金(81172253);上海市科委浦江人才计划基金(10PJ1407100);上海交通大学医学院附属仁济医院重点学科项目(RJ4101306)资助

摘　　要：目的:研究糖酵解抑制剂2-脱氧-D-葡萄糖(2-deoxy-D-glucose,2-DG)对人类非霍奇金淋巴瘤细胞株Namalwa、SU-DHL-4的作用效应及机制。方法:采用锥虫蓝拒染法检测细胞增殖,D-葡萄糖[HK法]检测试剂盒检测葡萄糖浓度,乳酸测试盒检测糖酵解途径终产物乳酸含量,反转录实时定量PCR(RTQ-PCR)检测糖酵解途径关键酶基因的转录水平,流式细胞术检测细胞周期。以健康人外周血梯度离心法分离所得的淋巴细胞为正常对照组。结果:淋巴瘤细胞株Namalwa和SU-DHL-4中糖酵解相关基因HK1、LDHA、GLUT1、HIF-1α在转录水平明显高于正常对照。2-DG能抑制淋巴瘤细胞株葡萄糖消耗,减少乳酸生成,将细胞周期阻滞于G0/G1期,致使细胞增殖受抑。结论:淋巴瘤细胞株Namalwa、SU-DHL-4存在糖酵解异常,2-DG通过抑制淋巴瘤细胞株糖酵解通路,可干扰细胞能量代谢,阻断细胞周期,使细胞增殖受抑,从而发挥抗肿瘤作用。Objective To investigate the effect of glycolytic inhibitor 2-deoxy-D-glucose（2-DG） on non-Hodgkin′s lymphoma（NHL） cell lines Namalwa and SU-DHL-4.Methods Cell proliferation was assessed by triplicate counting of trypan blue dye-excluding cells.Glucose consumption was assessed by Glucose（HK） Assay Kit before and after the treatment of 2-DG.Concentration of lactic acid,the final metabolic product of glycolysis pathway,was assayed by Lactic Acid Detection Kit.Furthermore,the expression of glycolysis related genes was determined by real-time quantitative PCR.Cell cycles were detected by flow cytometry.Results Glycolysis related genes were up-regulated in Namalwa and SU-DHL-4 cell lines when compared with normal lymphocytes.Cell proliferation,glucose consumption,lactic acid generation and proportion of cells in S-phase were inhibited by 2-DG.Conclusions Glycolysis pathway plays an important role in cell proliferation of NHL cell lines,and inhibition of this pathway may provide a new therapeutic strategy in the treatment of NHL.

关 键 词：非霍奇金淋巴瘤 糖酵解 2-deoxy-D-glucose Namalwa SU-DHL-4 

分 类 号：R733[医药卫生—肿瘤]