基于免疫胶体金试纸条检测盐酸克伦特罗的便携式光电型传感器  

A Portable Photoelectric Sensor Based on Colloidal Gold Immunochromatogrphic Strips for Rapid Determination of Clenbuterol in Pig Urine

作　　者：张洪才[1] 刘春燕[1] 刘国艳[1,2] 陈小连[1,2] 叶雨丹 王艺如[1] 柴春彦[1,2] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海市兽医生物技术重点实验室,上海200240

出　　处：《分析化学》2012年第6期852-856,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目;上海市兽医生物技术重点实验室开放课题基金资助

摘　　要：利用免疫竞争抗制原理制备检测盐酸克伦特罗的免疫纳米金试纸条,盐酸克伦特罗与CLB-BSA复合物竞争性结合胶体金上抗体的有限位点,从而使试纸条上的检测带显色减弱或消失。将显色后的试纸条插入自行研制的光电型传感器的测试孔内,根据反射光的强弱计算出克伦特罗的浓度。结果表明:用光电传感方法检测盐酸克伦特罗的线性范围为1～10μg/L,检出限为0.05μg/L,回收率为85.5%～96.0%。此光电型传感器检测克伦特罗具有简便、快速、灵敏、特异性强的优点。Colloidal gold immunochromatography strips for the determination of clenbuterol（CLB） were developed based on the principle of immune competitive inhibition.Clenbuterol competes the specific combining site on the CLB antibody with the conjugated compound of clenbuterol and bovine serum albumin（BSA） immobilized on the strip as the testing dot,resulting in the decreased pigmentation or disappearance of the testing dot on the strip.The sensing system was easily established by inserting the test strip into the slot of the portable photoelectric sensor.The concentration of CLB is obtained from the calculation of the intensity of the reflected light.The results showed that the response of the photoelectric sensor to concentration of clenbuterol displayed a linear correlation over a range from 1 to 10 μg/L with a detection limit of 0.05 μg/L,and the recovery reached 85.5%-96.0% in the pig urine samples.The photoelectric sensor based on the colloidal gold immunochromatography strips for the detection of clenbuterol demonstrated the advantage of easy operation,short detecting time,favorable sensitivity and specificity.

关 键 词：盐酸克伦特罗 光电型传感器 免疫胶体金试纸条 猪尿 

分 类 号：TS207.3[轻工技术与工程—食品科学]